FIRST SCHEDULE [r. 2]
FORMS
_______________ | |||
MINISTRY OF HEALTH | |||
_______________ | |||
THE KENYA MEDICAL LABORATORY TECHNICIANS AND TECHNOLOGISTS BOARD | |||
CURRICULUM FOR CERTIFICATE IN MEDICAL LABORATORY SCIENCES |
Year | On-Campus | Clinical Attachment Hours | |
I | TERM ONE | 440 | - |
TERM TWO | 440 | - | |
TERM THREE | 440 | - | |
2 | TERM FOUR | - | 440 |
TERM FIVE | - | 440 | |
TERM SIX | 440 | - | |
TOTAL | 1760 | 880 |
a) Project | 60 hours | |
b) Practicals and Orals | 4 hours | |
c) Theory | 2 hours each |
Grade | Score% | |||
A | 75-100 | |||
B | 65-74 | |||
C | 50-64 | |||
D | 40-49 | |||
E | 0-39 |
Quantitative Analysis | • Definition of qualitative analysis. • Concentration terms. • Preparation of solutions. • Acid/base indicators. • Glassware used in quantitative measurements. |
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Organic chemistry | • Terms used. • Difference between saturated and unsaturated compounds. • Homologous series. • Common uses. Alkanes Alcohol. Aldehydes. Ketones. Carboxylic acids. |
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Physical and chemical changes |
• Physical changes. • Chemical changes. |
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Atom, elements, compound and mixtures | • Structure of an atom properties of an atom. • Dalton’s Atomic Theory. • Mixtures and compounds. • The periodic table. • Relationship of physical and chemical properties and their position in the periodic table. • Relationship of physical and chemical properties of elements in the periodic table. |
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Chemical combinations | • Types of bonds. • Chemical equations. • Properties of bonds. |
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Acid, bases and salts | • Definitions. • Properties. • Differences between weak and strong acids and bases. • pH scale. • Neutralization. • Salts. |
a) Computers: | -Personal computers. | ||
-Micro-computers. | |||
Component's of a computer: | -Hardware and software | ||
-Hardware: CPU, Input and Output devices, files storage devices. | |||
Software-operating system - | Application programmes. | ||
2) Principles of computer operating system: | |||
• OS. | |||
• Application programmes | |||
-Major applications | |||
• Data Management: - | Person's role to assure correct data | ||
-Computer environment — Assuring power supply. | |||
-Introduction to windows — Word Processing. | |||
Setting up files. | |||
Modifying, storing and Laboratory Management. |
Topic | Sub Topic | Time | |
ENTREPRENEURSHIP AND SELF-EMPLOYMENT | • Importance of self-employment. • Entrepreneurship contribution to National development. • Requirements for entry into self-employment. |
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ENTREPRENEURIAL OPPORTUNITIES | • Business opportunities. • Assessing product demand. • Matching skills and resources to changing technology. |
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ENTREPRENEURIAL AWARENESS |
• Evaluating business environment. • Type of business finance. • Contractual agreements. • Government policy on small scale enterprises. • Problems of starting a business enterprise. |
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ENTREPRENEURIAL MOTIVATION |
• Internal motivating factors. • Techniques of self assessment. • External motivating factors. |
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ENTREPRENEURIAL COMPETENCE | • Decision making in business. • Institute change. • Coping with competition. • Risk taking. • Techniques of time management. • Leadership qualities. |
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ENTERPRISE MANAGEMENT | • Evaluating business goals. • Efficiency of resources utilization. • Finance planning. • Production management. • Management of human resources. • Work study. • Marketing and public relations. • Information management. • Project planning. |
Topic | Sub-Topic | Time | |||
i) Introduction to Anatomy and physiology | Definition | ||||
Importance | |||||
Cells | Structure | ||||
Functions | |||||
Epithelial cells: | Definition | ||||
Types, structure and | |||||
Sites | |||||
Tissue Types | |||||
Organs Structures | |||||
Systems Functions | |||||
ii) Circulatory system | Blood, the heart, blood vessels and sketch of these structures. | ||||
iii) Urino-genital-system | The kidney and urino-genital tract, the reproductive organs, sketch of their structures. | ||||
iv) Digestive system | The stomach, the liver, intestines, pancreas and their sketches.· |
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v) Respiratory system | The nose, trachea, and lungs and their sketches. | ||||
vi) Practical | Identification of various cells, tissues, organs and systems. |
Topic | Sub-Topic | |||||
1 | Lab-instruments | Colorimeter flame photometer oven incubators microscopes urinometers centrifuge ISE Deep freezers Refrigerators glucometer stills balances. | ||||
Apparatus | Dilutors Dispensers Lab wareIntegral syringe. | |||||
2 | Instrument installation | Size of instrument weight voltage ventilation. | ||||
3 | Bench Organization | Water volatile chemicals fumes fire outbreak biowaste. | ||||
4 | Principles of functional units | • Photometry: colorimeter Flame photometer Glucometer, ELISA • Heating Elements: Water bath Incubators Hot air Autoclave Stills Incinerators. • Microscopy Miscropes-light- inverted • Photoelectric • Centrifugal forces: Centrifuges • Refrigeration Deep freezers refrigerators cold room. • Density Urinometer • Measurement Weight- Balance Volumes Dilutors Dispensers Integral syringes and reagent bottles. • Electrochemistry: Ion selective electrodes Deionizers. pH meter |
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5 | Daily maintenance • Instruments • Apparatus • Decontamination |
• Dusting covering cleaning of instruments daily checks and servicing visits trouble shooting. • Cleaning drying • Disinfectant anti-septic sterilization. |
Code | Topic | Sub-Topic | Time | ||
Laboratory Layout |
• Draw a simple basic laboratory layout • Visit medical laboratories. |
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Safety | • Glass fittings. • Electrical connection heating. • Fire extinguishing and control. • Protective clothing. • Storage of chemicals, reagents and specimens cabinets. • Carrying transporting and mixing of chemicals and reagents. • Labeling classification. |
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Cleanliness | • Cleaning of benches, floor, sink, glassware, plastic ware and procedures involved. | ||||
First Aid | • Definition, aims and roles of first aid. • Assessment of accident situation. • Management of clinical conditions requiring first aid. • Ethics in first aid. • Demonstrations from St. Johns Ambulance on first aid techniques. |
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Management | • Inventory and purchasing. • Recording information. • Stocktaking. • Preparation of purchase documents. |
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Communication | • Communication. • Skills. • Implementing storage and retrieval. |
Topic | Sub-topic | ||
1. ALGEBRA | • Indices. • Logarithms. • Applications of logarithms. • Linear equations. • Simultaneous equations. • Matrices. • Transposition of formulae. |
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2. QUADRATIC EQUATIONS | • Solutions. • Applications. |
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3. LINEAR AND NON-LINEAR GRAPHS | • Construction. • Solutions. |
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4. COLLECTION, ORGANIZATION AND PRESENTATION OF DATA |
•Data collection. • Data organization. • Date presentation. |
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5. DATA ANALYSIS | • Measures of central tendency. • Measures of dispersion. |
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6. SIMPLE REGRESSION AND CORRELATION ANALYSIS |
• Regression analysis equivalent 2 variables only. • Correlation analysis 2 variables only. |
Topic | Sub-Topic | ||
Introduction | • Projects. | ||
Data collection | • Observational method. • Interviews and Questionnaires. • Trace measures. • Content analysis. • Data achieves. • Measurements. • Qualitative method. ° Data representation. ° Central tendency. ° Dispersion. ° Regression analysis. |
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Use of computer | • Application of spreadsheets to compiling data. • Production of report. |
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Project write up | • Documentation of sources. • Carrying out of project. • Reporting ° Lay out. ° Data presentation. |
Topic | Sub-Topic | Time | |
1. SOCIAL STUDIES | • Basic medical psychology. • Basic medical sociology. • Social economics. • Government. • National Philosophy. • Science and technology. • Commerce. • Personal inter-relationships. |
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2. ETHICS | • Meaning and importance. • Role of religion on society. • Significance of social and individual values. • Constitution of Association of Kenya Medical Laboratory Scientific Officers. • Technology and religion. |
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3. LAW | • Definition. • Importance of law. • Sources of Kenyan laws Public Health Act. • Medical Laboratories Technicians and Technologists Act. • Elements of law. • Law in day to-day life of an individual. |
Topic | Sub-Topic | Time | |
Terminologies | Sterilization, disinfection, germicides, bactericides, antiseptics, fungicides, bacteriostatics. |
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Methods | Physical methods: Heat, dry heat, moist heat. Radiation: Ultra-violet, ionisation radiation, filtration. Chemical methods: Alcohol, chloroform, chlorine, glycerol, phenol, cresol, aldehyde, quaternary ammonium compounds. |
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Factors influencing sterilisation | Nature, load and type of micro-organisms, nature of material and containers, time and temperature, humidity and organic contaminants. | ||
Sterility testing | Automatic process control, recording thermometer, thermocouple measurement, chemical indicators, adhesive tape (autoclave) biological control. |
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Sterilization, disinfection and waste disposal | Microbiology, clinical chemistry, haematology, blood transfusion, parasitology, histopathology. |
Year | Topic | Sub-Topic | ||
Introduction | Clinical Chemistry. Introduction, definition. Physical chemistry, definition of atoms, atomic structure, elements, molecules, compounds, nails micro and organic. Bases: Strong and weak. pH: pH scale. Calculations. Preparation and importance. Indicators: Litmus methyl orange, red Phenolphthalein. Solutions: Standard working; saturated, supersaturated, normal, molar, solution, formula. RV x RC __________ OC Titration – Principle. Procedure. Chloride. Calculations. |
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Organic chemistry | Definition, structure of carbon, homologous series. | |||
Biochemistry | Definition, biomolecules, carbohydrates, amino acids, and proteins, lipids, vitamins, classifications. | |||
Basic physiology | Functions of the body systems: • Kidney. • Liver. • Pancreas, • Stomach, intestines. • Capillaries, arteries, veins. |
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Basic pathology | The liver in relation to bilirubin, pancreas in relation to diabetes nephrosis. | |||
Safety measures— chemicals | Sources of injuries Cacinogenic poisonous, radioactive, explosives, fuming. Protective measures: Protective gear. Methods of disposal. Decontamination. |
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Instruments | Types of injuries: • Mechanical, electric, thermal (hot water, hot air, steam, dry heat. Protective measures • Protective gear. • Bench organization. • Proper insulation and voltage. |
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Lab. ware | Source of injuries. Breakages, sharps. Mechanical. Protective measures. Protective gear, proper handling and disposal. |
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Maintenance and care of Lab. ware | Glassware, plastics, ceramics. Cleaning: use of detergents, dichromate solution, strong acids and hot water. Drying: room temperature, hot air oven. Storage, racks, canisters, drawers, cabinets. |
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Instruments | Daily maintenance: checks, manufacturer instructions. Laboratory, organization, instrument installation and regular servicing. |
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STORAGE OF CHEMICALS AND REAGENTS |
Corrosives: non-metallic containers, labelling, isolation, refrigeration expiry date. Volatile and flammables cold storage. Ventilation, isolation, fireproofing, hazard labels. Analytical reagents and chemicals labelling, aluminium foils and lead containers for radioactive material. Lockable cabinets, desiccated cabinets, brown containers, and dark rooms. Labelling to include expiry dates. |
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Specimen Collection | Containers, anticoagulants, disposable needles, and syringes. Labels, preservatives, request form interpretation. | |||
Mode of Collection | Aseptic technique, hygienic. Sites of bleeding and stasis. Types of specimen. Blood, stool, urine, C.S.F. Aspirates and exudates saliva, sweat. |
Year | Topic | Content | ||
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Basic principles Pipettes: • Types • Pipetting |
• Graduated • Volumetric. • Pasteur. • Micropipettes. • Automated. • Mouth. • Fillers. • Capillarity. • Atmospheric pressure. |
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Qualitative and quantitative | Urine – physical examination. • Chemical analysis. • Microscopy. Stool – Physical examination. • Chemical analysis. C.S.F. – Physical examination. • Biochemistry. Urine sugars. • Proteins. • Clearance tests. • Osmolarity. • pH. Blood – Glucose. • Urea. • Bilirubin. • Creatinine. • Electrolytes. • Uric acid. • Protein. • Transaminases. • Alkaline phosphatase. |
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Separation | CSF exudate aspirates. • Glucose. • Protein. Urine sugars – Chromatography. |
Year Two | Topic | Content | ||
Clinical placement | Clinical placement. | |||
Practicals Urine Qualitative and quantitative |
Volume, colour, appearance, odour, sugars, ketones, bilirubin, blood, protein, pH, crystals, casts, cells, clearance, osmolarity | |||
Blood Quantitative |
Glucose, urea, creatinine, electrolytes, uric acid, total protein, albumin, transaminases, alkaline, phosphatase. | |||
CSF, Exudates and aspirates. | Glucose, protein. | |||
Saliva and sweat | Enzymes and electrolytes. | |||
Separation | Urine sugars and reducing substances (chromatographic techniques). | |||
Quality control measures | Handling of control materials levy, Jennings plots, units in chemical pathology and reference ranges. |
Topic | Sub-Topic | Time | ||
IT | INTRODUCTION | • Importance of Histopathology and cytology. •Tenninologies used. • Sources of specimens. |
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2T | CELL AND EPITIIELIUM | • Cell structure and division. • The four primary tissues. • Types of epithelial cells. • Function of epithelial tissues. |
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3T | FIXATION AND FIXATIVES | • Purposes of fixation. • Tenninologies used. • Effects of fixatives. • Preparation. • Methods of.fixation. • Storage and labelling. |
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4T | DECALCIFICATION | • Definition. • Methods of decalcification. • Treatment of tissue after decalcification. • Determination end point of decalcification. |
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5T | TISSUE PROCESSING | • Dehydration. • Clearing. • Wax impregnation and other common embedding media. • Storage of blocks slides and reports. |
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6T | MICROTOMY | • Types of microtomes. • Microtomes knives. • Knife sharpeners. • Faults in sectioning. • Floating out of sections. |
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7T | SECTION ADHESIVES | • Types of adhesives. • Purpose of adhesives. • Preparation. |
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8T | THEROY OF STAINING | • Definition of dyes and stains. • Preparation of stains. • Types of staining reactions. • Staining methods. • Staining equipment. |
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9T | HISTOLOGICAL PIGMENTS | • Definition. • Types of pigments encountered. • Their identification and removal. |
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10T | CYTOLOGY | • Definition. • Terminologies used. • Uses of cytology. • Sources of specimens and collection. •Equipments/apparatus used. • Fixatives employed. • Staining methods. • Slide screening. |
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11T | MOUNTANTS | • Types of mountants. • Uses of mounting media. • Methods of mounting. • Ringing media. |
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12T | MUSEUM TECHNIQUES | • Collection of specimens. • Methods of preservation. • Labeling and display of specimens |
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13T | SAFETY PRECAUTIONS | • Physical injuries. • Fire hazards. • Chemicals. • Explosives. • Infectious specimens. |
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14T | MORTUARY TECHNIQUES | • Public relations. • Cultural values. • Body handling. • Body dressing. • Basic embalming. • Body suturing. |
Year | Topic | Sub-topic | Theory (T) practice (P) |
Hours |
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ONE | INTRODUCTION TO VIROLOGY |
* DEFINITION OF VIRUSES. * GENERAL PROPERTIES OF VIRUSES. *CLASSIFICATION OF VIRUSES (CRITERIA). |
T T |
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BIO-SAFETY | CATEGORISATION OF PATHOGEN RISK GROUPS. * ACTIVITIES HARMFUL TO THE WORKER AND OTHERS IN VIROLOGY. * OCCURANCE OF LABORATORY INFECTIONS AND THEIR PREVENTION. * MODE OF INFECTIONS IN AND OUT OF THE LABORATORY. * LOCATION OF HEALTH AND SAFETY EQUIPMENT IN THE WORK PLACE (EG. FIRE EXTINGUISHERS). PERSONAL PROTECTION: USE OF SAFETY-GEARS, EG. LAB. GOWNS, GLOVES, MASKS AND GOGGLES. * USE OF PIPETTING AIDS. * USE OF SAFETY CABINETS. BIO-SAFETY * SAFE USE OF OTHER EQUIPMENT, DEFINITIONS AND TYPES. |
T T T T T |
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STERILIZATION | METHODS OF STERILISATION, FACTORS INFLUENCING STERILIZATION AND STERILITY TESTING |
P | ||
DISINFECTION AND DISPOSAL |
DISINFECTIONS; ‘CIDAL’ AND ‘STATIC’ DISINFECTANTS. * MODE OF ACTION OF DISINFECTANTS. * COMMON DISINFECTANTS AND THEIR USE. DILUTIONS. DISPOSAL: DISINFECTION AND METHODS. |
T T |
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EQUIPMENT | USE OF THE FOLLOWING IN VIROLOGY: EQUIPMENT INVERTED MICROSCOPE AUTOCLAVE. |
T P |
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SPECIMEN | WATER BATHS, DEEP FREEZERS, REFRIGERATORS, INCUBATORS, BIOSAFETY CABINETS. |
T P |
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STERILIZATION | PRACTICAL SPECIMEN, COLLECTION, HANDLING, TRANSPORTATION, PRESERVATION AND STORAGE. PRACTICAL METHODS: MOIST HEAT, DRY HEAT, CHEMICAL, STERILIZATION DISINFECTANTS. |
P |
(1) Autoclave (portable) | 1 between 10 students. | ||
(2) Medium water bath | 1 between 5 students | ||
(4) pH meters | 1 between 5 students | ||
(5) Anaerobic jars | 1 between 5 students | ||
(6) Incubators/Hot air oven (adjustable) | 1 between 10 students | ||
(7) Distillers | 2 for the whole institute | ||
(8) De-ionizers | two small | ||
(9) Microscopes binocular | 1 between 10 students | ||
(10) Weighing balance | 1 top pan load balance | ||
(11) Wood lamp | 1 | ||
(12) Centrifuge | 1 between 4 students | ||
(13) Bunsen Burner/Spirit | 1 between 2 students | ||
(14) Tripod Stands/asbestos mat | 1 between 10 students | ||
(15) Fridge/deep freezer | 1 between 10 students | ||
(16) Safety Cabinet | 1 per laboratory | ||
(17) Teaching microscopes | 1 between 10 students | ||
(18) Mechanical shaker | 1 between 10 students. | ||
(19) Inoculating loops | 1 per student | ||
(20) Assorted microbiology glassware | adequate | ||
31.0.2 CLINICAL CHEMISTRY | |||
(1) Colorimeters | 1 between 4 students | ||
(2) Analytical balance0150 – top pan loading | |||
(3) Sensitivity up to 1mg. | 1 between 5 students | ||
(4) Flame photometers | 1 between 10 students | ||
(5) Centrifuge | 1 between 4 students | ||
(6) Refrigerators/Freezers | 1 between 10 students | ||
(7) Water bath medium | 1 between 4 students | ||
(8) pH meter | 1 between 5 students | ||
(9) Mechanical mixers | 2 | ||
(10) Electrophoresis equipment | 2 per institution/class | ||
(11) Distiller/deionizer | 2 | ||
(12) Hot air oven/incubator adjustable | 10 | ||
31.0.3 HAEMATOLOGY | |||
(1) Haemoglobinemter | 1 | ||
(2) Centrifuge | 1 | ||
(3) Microhaematocrit centrifuge | 1 between 5 students | ||
(4) Blood mixers rollers | 1 between 10 students | ||
(5) Water bath | 1 between 10 students | ||
(6) Incubator | 1 between 10 students | ||
(7) Colorimeter | 1 between 10 students | ||
(8) Electrophoresis equipment | 1 between 10 students | ||
(9) Sphygmomanometer | 1 between 5 students | ||
(10) E.S.T. stands | 1 between 4 students | ||
(11) Deep freezer/fridge | 1 per 10 students | ||
(12) Deep freezer | 1 between 5 students | ||
(13) Coulter counter | 1 for each class | ||
(14) Neubaeur Chambers | 1 for each student | ||
(15) Distiller | 2 per institution/class | ||
(16) Analytical balance | 1 between 10 | ||
31.0.4 BLOOD TRANSFUSION SCIENCES | |||
(1) Blood bank refrigerator | 1 per class/institution | ||
(2) Grouping tiles | 1 per student | ||
(3) Water bath adjustable | Medium size | ||
(4) Plasma extractors | 15 students | ||
(5) Centrifuges | 1 between 4 students | ||
(6) Weighing balance | 1 between 5 students | ||
(7) Syphmomanometers | 1 between 5 students | ||
(8) Hot air oven (adjustable) | 1 in the whole institution | ||
(9) De-ionizers and stillers | 1 for the whole class/institution | ||
(10) Mechanical shaker | |||
(11) Blood transfusion bleeding unit | |||
(12) Assorted blood transfusion glassware and adequate apparatus |
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(13) Microscopes | 1 per 2 students | ||
31.0.5 HISTOPATHOLOGY | |||
(1) Microtome rocking/rotary | 1 per 4 students | ||
(2) Manual tissue processing set | 1 between 4 students | ||
(3) Hot plat | 1 between 4 students | ||
(4) Hone and strope | 1 between 4 students | ||
(5) Automatic knife sharpener | 1 per class/institution | ||
(6) Water bath, medium size | 1 between 4 students | ||
(7) Microscope (teaching) | 1 for the institution | ||
(8) Cold plate | 1 between 6 students | ||
(9) Weighing balances | 1 between 5 students | ||
(10) De-ionizers | 1 per class/institution | ||
(11) Fume chamber | 1 per laboratory/institution | ||
31.0.6 PARASITOLOGY | |||
(1) Microscopes | 1 for 4 students | ||
(2) Centrifuges | 1 for 4 students | ||
(3) Refrigerators | 1 per student | ||
(4) Pestle and mortar | 1 for 4 students |
Title | Author | |||
I. | Introduction to Medical Laboratory Technology | F.J. Baker & Silverton. Current Edition | ||
2. | Medical Laboratory Manual for Tropical Countries Part I & II |
Monica Chesbourogh |
Title | Author | |||
1. | Colour Atlas and Text Book of Diagnostic Microbiology |
Elmer W. Koneman et al | ||
2. | Short Text book of Microbiology | Satish Gupte |
Title | Author | |||
1. | A handbook of Clinical Chemistry | V.W. Sitati | ||
2. | Practical Clinical Biochemistry | Harold V. Valley | ||
3. | Essential of volumetric Analysis | By J. Lambert |
Title | Author | |||
1. | A short textbook of haematology | R.B.Thomson | ||
2. | Atlas of haematology | McDonald Dodds | ||
3. | Practical Haematology | Dacie &Lewis |
Title | Author | |||
I. | Carlton's Histological Techniques | Drowry and Wellington | ||
2. | Colour atlas | lrwing Bernem | ||
3. | Theory and practice of histological Techniques | John Bancroft |
Title | Author | |||
1. | Carlton's Histological Techniques | Drowry and Wellington | ||
2. | Colour atlas | Irwing Bernem | ||
3. | Theory and practice of histological Techniques | John Bancroft |
Title | Author | |||
1. | Basic Clinical Parasitology | Harold W. Brown | ||
2. | Introduction to Parasitology | A.C. Chandler | ||
3. | Atlas of Helminthology Protozoology | Leach | ||
4. | Lecture notes on Medical Entomology | M.W. Service | ||
5. | Tropical Diseases | Manson Barr |
Title | Author | |||
1. | Practical virology for Medical Students and Practitioners in tropical countries |
D. Metasalaar et al | ||
2. | Fundamentals of Medical Virology | Kucera and Louis S. | ||
3. | Virological Procedures | Hopkins et al | ||
4. | Virology - Practical Approach | B.S. Mahy etal | ||
5. | Medical virology | D. White & F. Fenner | ||
6. | Medical Virology- A Practical Approach | Editor - U. Desselberger | ||
7. | Principles of Molecular Virology | A.J. Cann |
Title | Author | |||
I. | The Principles of Immunology | Ivan Roitt | ||
2. | Fundamentals of Immunology | Tesdale | ||
3. | Practical Immunology | Hudsons and Hay | ||
4. | Practical Immunology | Talwar | ||
5. | Basic and Clinical Immunology | Peakman & Vergains | ||
6. | Understanding Immunology | Peter Woods & Prentice Hall |
SECOND SCHEDULE [r. 2]
DIPLOMA CURRICULUM
MINISTRY OF HEALTH
THE KENYA MEDICAL LABORATORY
TECHNICIANS AND TECHNOLOGISTS BOARD
CURRICULUM
FOR DIPLOMA
IN
MEDICAL LABORATORY SCIENCES
TABLE OF CONTENTS
Course Title1.0Introduction.2.0Rationale.3.0Roles and functions.4.0Programme aim.5.0Programme objectives.6.0Admission requirements.7.0Course duration.8.0Attendance pattern.9.0Award of certificates.10.0Teaching methods.11.0Teaching aids and resources.12.0Resources.13.0Format of students assessment and evaluation.Chemistry.Computers.Entrepreneurship education.Instrumentation.Health management.Management and laboratory practice.Mathematics and statistics.Medical terminologies.Research methods and projects.Social studies, professional conduct, ethics and law. Sterilization and disinfection.Medical microbiology.Clinical chemistry.Haematology.Histopathology and cytopathology.Blood transfusion science.Medical parasitology.Protozoology.Helminthology.Medical entomology.Cestodes.Malacology.Mammology.Insecticides.Virology.Immunology.Appendix 1: Training standards.Appendix 2: Essential equipment.Appendix 3: Essential books.___________________________
COURSE TITLE
DIPLOMA IN MEDICAL LABORATORY SCIENCES
1.0INTRODUCTIONThis course is intended to equip the trainees with knowledge, skills and attitudes to enable them to work as medical laboratory technologists.2.0RATIONALEThe public has become more aware of their health needs hence increasing the demand for laboratory services, which also includes use of technology and techniques that were not available previously. Therefore the course aims at providing health care professionals who will serve at Class B Laboratory level at both the public and private sector.3.0ROLES AND FUNCTIONS(i)Carry out laboratory tests.(ii)Analyse, interpret and report laboratory results.(iii)Manage laboratory resources.(iv)Initiate and participate in the improvement of diagnostic procedures.(v)Create awareness and appreciation of laboratory services to the general public.(vi)Plan and set up a laboratory.(vii)Participate in the training of laboratory personnel.(viii)Supervise other laboratory personnel.(ix)Carry out quality assurance and quality control.(x)Participate in continuing medical education.4.0PROGRAMME AIMThe course is intended to equip trainees with knowledge, skills and attitudes that will enable them to provide health services at Class B Laboratory level as medical laboratory technologists.5.0PROGRAMME OBJECTIVESAt the end of the course the trainee should be able to:(i)Apply the principles that form the basis of medical laboratory.(ii)Apply laboratory practice.(iii)Practise safety precautions in a medical laboratory.(iv)Select, set up and operate laboratory equipment.(v)Carry out standard procedures to obtain quality results.(vi)Interpret laboratory results.(vii)Carry out research.(viii)Manage service and resources of a medical laboratory.(ix)Participate in the multi-disciplinary planning, implementation, co-ordination and evaluation of healthcare of the individual and community.(x)Contribute to the development of science and technology through creativity and application of acquired knowledge, skills and attitudes.(xi)Demonstrate the ability to evaluate own performance.(xii)Plan continuing education for self and others.(xiii)Observe the professional code of conduct and ethics.6.0ADMISSION REQUIREMENTSTrainees entering this course should have the following minimum requirements obtained at one sitting:—Kenya Certificate of Secondary Education (K.C.S.E.) with a mean grade of C, or equivalent, and in addition a minimum grade of C in the following:—Biology/Biological Sciences Chemistry/Physical Sciences English or Kiswahili They should also have a minimum grade of C in the following:—Mathematics or PhysicsOR
Holders of Certificate in Medical Laboratory Sciences with two (2) years relevant experience.7.0COURSE DURATIONThe course is designed to have duration of 3960 contact hours where 1930 hours are spent on campus and 1760 hours are spent on clinical placement.8.0ATTENDANCE PATTERNThe course is designed to be covered as follows—Year | On Campus | Clinical Placement | |
TERM I | 440 | – | |
TERM II | 440 | – | |
TERM III | 440 | – | |
TERM IV | – | 440 | |
TERM V | – | 440 | |
TERM VI | 440 | – | |
TERM VII | – | 440 | |
TERM VIII | – | 440 | |
TERM IX | 440 | – |
Project Practicals and orals Theory |
60 hours 3 hours each 2 hours each |
Alternative A: | Section A M.C.Q. (40 marks) 15 short, structured questions Section B (30 marks) Section C (30 marks) Two out of three attempted |
Alternative B: | Section A (40 marks) 15 short, structured questions Section B (60 marks) Three out of five attempted (long and short) |
Grade | Score by % | ||
A | 75–100 | ||
B | 65–75 | ||
C | 50–64 | ||
D | 40–49 | ||
E | 1–39 |
Code | Topic | Sub-topic | Time | ||
Quantitative analysis | • Definition of qualitative analysis, terms used in chromatography. • Concentration terms. • Preparation of solutions. • Acid/base indicators. • Glassware used in quantitative measurements. |
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Organic chemistry | • Terms used. • Difference between saturated and unsaturated compounds. • Homologous series. Common used. Alkanes. Aldehydes. Ketones. Carboxylic acids. Amines. |
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Physical and chemical changes |
• Physical changes • Chemical changes. |
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Atom, elements, compounds and mixtures |
• Structure of an atom. • Preparation of an atom. • Dalton’s Atomic Theory. Mixture and compounds. The Periodic Table. Relationship of atom structure of an element to its position in the Periodic Table. Relationship of physical and chemical properties of elements in the Periodic Table. |
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Chemical combinations | • Types of bonds. • Chemical equations. • Properties of bonds. |
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Acid, bases and salts | • Definition. • Properties. • Differences between weak and strong human acid and bases. • pH scale neutralisation. • Salts. |
Topic | Sub-topic | ||
Introduction to computers | Concept of computing. Components of a computer. Hardware – CPU (ALU and control units). • Main memory. • RAM. • ROM. • Auxiliary memory. • Peripherals. Software – OS and Applications. Interaction of hardware and OS. |
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Principles of computer operations | Computer capability Computer environment • Power assurance. • Dust. • Water leakages. • Temperature. • Humidity. Data – Definition of data and information. Data entry (capturing) • Optical. • Magnetic. • Sound (verbal). Data security • Data back-up. • Persons role to assure correct data. • Operating system. • Application compatibility. • Selection of applications. |
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Data management | • Introduction to windows. • Word processing: (1) Setting up files. (2) Modifying. (3) Storing. (4) Retrieval of information. (5) Printing. Spreadsheets— Types: Excel File set-up. |
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Data analysis | Data entry. Data modifications. Printing process. Basic statistical functions. Interpreting of the outputs. |
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Role of computers |
Role of computers in managing laboratory sciences. |
Topic | Sub-topics | Time | |
1. | INTRODUCTION | Entrepreneurs and entrepreneurship. Entrepreneur’s contribution to national development. Role of entrepreneurs in business. |
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2. | ENTREPRENEURIAL OPPORTUNITIES |
Generation of business ideas. Business opportunities. Selection of suitable market. Marketing. |
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3. | ENTREPRENEURIAL AW ARENESS |
Business information. Procedures in starting a business. Sources of finance. Factors considered when selecting sources of business finance. Legal aspects of a business enterprise. Government policy. Tendering. Business environment. Technology choice. Business ethics. |
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4. | ENTREPRENEURIAL MOTIVATION |
Characteristics of a successful entrepreneur. Self assessment of entrepreneurial potentials. Incentives for aspiring entrepreneurs. |
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5. | ENTREPRENEURIAL COMPETENCIES | Decision-making. Coping with change and competition. Risk-taking. Leadership. Communication. Time management. |
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6. | ENTERPRISE MANAGEMENT | Setting business objectives. Resources management. Financial management. Production planning. Public relations. |
Topic | Sub-topic | Contents |
Types of laboratory instruments | Instruments | Flame photometer, dissecting microscope, tissue processor, wax dispensers, staining processors, microtomes, paraffin wax oven, knife shapeners, photometers, flourimeters, ISE, pH meter elecrophoresis systems, chromatographic systems, oven incubators, centrifuges, refrigerators, balance, still, glucometers, incubators, autoclave, microscopes, vacuum embedding, cryostat ELISA readers, ELISA washers, mixers, rollers, urinometers, automation, biosafety cabinets sonic and ultrasonic macerators. |
Apparatus | • Fans, electric wiring, plasma extractors, dilutors, dispensers, laboratory ware, hand lenses, intergral syringes, stropes, dissecting kit. | |
Instrument Installation |
Dimensions Bench organization Measurements: Weight Volume Electro-chemistry Pressure |
Size of instrument, weight, voltages, ventilation. Safety from water, volatile chemical, fumes, fire outbreak, biowaste. Balances Dilutors, Dispensers Integral syringes and reagent bottles. Ion selective electrode, deionizers, pH meter. Vacuum embedding. |
Daily Maintenance |
Instruments: Pressure Decontamination: Measurements Weight Volume Electro-chemistry Pressure |
Dusting, covering, cleaning of instruments, daily checks, servicing visits, trouble shooting, greasing, defrosting. Cleaning, drying Disinfectants, anti-septics, sterilization Balances Dilutors, dispensary, integral syringes and reagent bottles. Ion selective electrode, deionizers, pH meter. Vacuum embedding autoclave. |
Daily maintenance | Instruments: Apparatus Decontamination |
Dusting, covering, cleaning of instruments, daily checks, servicing visits, trouble shooting, greasing, defrosting. Cleaning, drying Disinfectants, anti-septics, sterilization. |
Principles of functional units |
Photometry Heating elements Microscopy Refrigeration Density |
Colorimeter, spectrophotometer, random access, nephelometer, automation flame photometer, glucometers. Water bath, incubators, hot air oven, autoclaves, stills, incinerators. Automatic tissue processor, paraffin wax oven, float baths wax dispenser. Light, inverted, photoelectric fluorescent, dark ground, dissecting. Centrifuges, automatic stain processor, refrigerated centrifuge. Deep freezers, refrigerators, cold room cryostat. Urinometers. |
Topic | Sub-topic | Contents |
Heating Elements Microscopy Centrifugal forces Refrigeration Density Measurements Weight Volume Electro-chemistry Pressure |
Water bath, incubators, hot air oven, autoclaves, stills, incinerators, automatic tissue processor, paraffin wax oven, float baths, wax dispenser, light inverted, photoelectric fluorescent, dark ground, dissecting Centrifuges, automatic stain processor, refrigerated centrifuge Deep freezers, refrigerators, cold room, cryostat Urinometers Balances Dilutors, dispensers, integral syringes and reagents bottles Ion selective electrode, deionizers, pH meter, Vacuum embedding. |
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Daily maintenance | Instruments Apparatus Decontamination |
Dusting, covering, cleaning of instruments, daily checks, servicing visits, troubleshooting, greasing, defrosting. Cleaning, drying Disinfectants, anti-septics, sterilization. |
No. | Topic | Sub-topic | ||
1. | OVERVIEW OF MANAGEMENT | Role of managers in organizations. Process of management. Importance of management in organisations. |
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2. | TECHNIQUES OF ORGANIZING | Process of planning. Importance of planning. Techniques used in planning. Strategic planning. |
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3. | TECHNIQUES OF ORGANIZING ACTIVITY | Basis of organising activities. Circumstances of choosing basis. Merits and demerits of each base. |
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4. | TECHNIQUES OF CO-ORDINATION | Process of co-ordination. Role of manager in directing organisational activities. Techniques used in co-ordination. |
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5. | HUMAN RESOURCE CO-ORDINATION | Structuring the system (analyzing and designing jobs). Recruitment, selection, placement internal mobility. Separations, death, retirements or resignation. Performance appraisal. Preparing for advancement. |
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6. | MANAGEMENT OF CHANGE OF CONFLICTS |
Factors influencing organization chances. Causes of resistance to change. How to overcome employee resistance to change. Techniques of managing change. |
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7. | TECHNIQUES OF MANAGING TIME | Meaning of conflicts. Advantages and disadvantages of conflicts in an organization. Techniques of managing conflicts. |
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8. | STRESS MANAGEMENT | Meaning of stress. Causes of stress. Techniques of managing stress. |
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9. | TECHNIQUES OF CONTROL IN ORGANISATIONS | Importance and nature of control. Types of control systems. Control techniques. |
Code | Topic | Sub-topic | Time | ||
Laboratory design | Setting of the laboratory. Layout/floor plan. Bench types. Sinks and drainage. Floor surfaces. Types of ventilation. Lighting in the laboratory. Conversion of an existing building to laboratory. |
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Safety precautions | Sources of danger in the laboratory and their prevention. Lab operation. Chemicals. Biological materials. Fires. Explosions. Gas cylinders. Electricity. Radiation. Use of protective clothing. Handling procedures. Choice of laboratory materials. Regulations. Role of supervisor. |
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Care for laboratory equipment | Spectrophotometers. Refrigerators. Microscopes. Incubators ovens. Water baths. Distillers and deionizers. Balances. Flame photometers. ELISA equipment. pH meters. Electrophoresis equipment. Microtomes. |
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Management | Explanation of management. Management and schools of thought. Principals of management. Planning and forecasting. Organizing. Controlling. Leading. Directing staffing. Co-ordinating. Motivating. |
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Organization | Structure. Principles of organization. Relationship. Delegation. |
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Management styles | Management by objectives. Management by exceptions. Management by crisis. |
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Materials control | Sources of information. Purchasing procedures. Receiving procedure. Types of storekeeper. Store documents. Control of stock levels. Security and protection of materials. |
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Laboratory records | Methods of storing and retrieving. Setting up protection of materials. |
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Communication | Purpose of communication. Process of communication. Informal and formal. Methods of transmission. Forms of written communication. Procedures used in planning and conducting. Interviews and meetings. Report writing— 1. General, 2. Technical. External communication. Mass media as a form. |
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First-aid | Definition, aims and roles of first-aid. Assessment of accident situations. Clinical conditions requiring first-aid. First-aid. Ethics in first-aid. Demonstrations from St. John on first-aid and techniques. |
Topic | Sub-topic | ||||
1. | The scientific calculator, use of. | ||||
2. | NUMBERS | Decimals. Fractions. Rounding. Standard form. |
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3. | ALGEBRA | Algebraic expressions. Equations – linear; simultaneous. Quadratic— indices and logarithms— exponential and log, equations. |
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4. | GRAPHS | Straight line graphs. Curves. Exponential. Deduction of laws to linear form. Gradients intercepts. |
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5. | RATIO, PROPORTION, PERCENTAGE AND VARIATION | Ratio. Proportion/Variation. Inverse, direct partial and joint. Percentage. |
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6. | MEASURING | Systems, conversions of units. Area, calculation of volume. |
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7. | DIFFERENTIATION AND INTEGRATION | Introduction of differentiation. Introduction of integration. Applications of differentiation and integration. |
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8. | STATISTICS | Collection of data. Organization of data. Representation of data. Statistical measures. Mean. Median. Mode. Standard deviation. Interpretation of data. Introduction. Simple regression and correlation. Analysis. |
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9. | PROBABILITY | Definition of probability. Classical definition. Axiomatic definition. |
Topic | Sub-topic | |||
1. | Introduction to medical terminologies | History and origin of medical terms. Qualities of medical languages. Principles of derived from Latin and Greek languages without alterations or modified to improve accuracy by addition of:— • “oid”, •“iform”, • prefixes such as “para” and “pseudo”. |
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2. | Medical words and terms | Words roots, prefix, suffix, combining forms. Compound words. Greek and Latin. Anatomical synonyms. |
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3. | Greek alphabets | List Greek alphabets and their meaning. | ||
4. | Application of medical words and terms | Resemblance – words derived with little or no alterations. Prefix “pseudo” (meaning an example). Words pertaining to— Cavities, membranes and partitions. Opening and communications— – fluids and substance quantity, – deficiency. Deficiency. Excess numbers. Paired and unpaired. Measurements and size. Textures and fabrics. Air and breadth. Form and sharp. Color. Hardness and softness. Thickness and weight. Surface identity relations. Age. Positions and relative arrangements and distributions. |
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Approximation and separation. Visibility. Temperature. Time. Goodness and badness. Ease and difficulty. Movement and transport. Sensation, feeling and affection. Growth and reproduction. Nutrition, digestion and excretions. Special service, cutaneous sensation. Mental states construction. Destruction and obstruction. Protection. Wasting, decay and death. Entomological terms. |
Topics | Sub-topic | ||
Introduction | – Hypothesis, research questions, objectives. – Ethical considerations. |
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Population and Sampling | – Population. – Types of sampling. – Probability sampling methods. – Non-probability sampling methods. |
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Data collection | – Observation methods. – Interviews and questionnaires. – Trace measures. – Content analysis. – Data archives. – Measurements. |
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Data analysis | – Qualitative methods. – Quantitative method (Statistics)— • binomial distribution. • poison distribution. • normal distribution. • student distribution. • estimation theory. • test of hypothesis in large and small samples. |
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Use of computer | – In statistical analysis. – In data organization. – Production of report. |
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Sampling frame | – Significance of sampling. – Methods of sampling— • probability samples, • non-probability samples. |
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Data collection | – Methods of collecting data— • Observation methods. • Interviews and questionnaires. • Trace measures. • Content analysis. • Data archives. • Measurements. |
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Data analysis | – Qualitative. – Quantitative analysis • binomial distribution. • poison distribution. • student distribution. |
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Test of hypothesis | – Estimation theory. – Test of hypothesis in large sample and small sample. |
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Computer applications | – Application of statistical computer packages for analysis. – Application of computers packers for data organization. |
Topics | Sub-topics | |||
1. | Social studies | – Medical psychology. – Medical sociology. – Basic economics – elements. – Social economics. – Government. – National philosophy. – Science and technology. – Commerce. – Personal inter-relationships. – Public relations. |
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2. | Ethics | – Meaning and importance. – Description of the role of religion influencing morality in society. – Significance of social and individuals. – Role of humanism in society. – Professional conduct and ethics. – Part VI of the Constitution of the Association of Kenya Medical Laboratory Scientific Officers. – Technology and religion. |
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3. | Law | – Definition. – Importance. – Sources of Kenyan Law – Constitution, Public Health Act Cap. 242 of the Law of Kenya, Medical laboratory Technicians and Technologists. – Cap. 253A. – Law of contract. – Law of tort. – Family law. – Land/real property –basic interests. – Law in the day to day life of an individual. |
Topic | Sub-topic | |||
(i) | Terminology | Germicides, disinfection, Bactericides, antiseptics, Fungicides, bacteriostats. |
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(ii) | Principles | Oxidation, Lysis (membrane disruption) denaturation of proteins, ionization and enzyme poisons. | ||
(iii) | Methods | Physical Heat: dry heat, moist heat. Radiation: Ultra-violet. Radiation: Ionizing. Filtration: Chemicals: Alcohol, chloroform, chlorine, glycerol, phenol, cresol, aldehydes, ethylene oxide, quarternary, ammonium compounds. |
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(iv) | Factors | Nature, local and type of Micro-organisms, nature of Material and containers, time, temperature, humidity, organic, contaminants. |
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(v) | Sterility testing | Automatic process control. Recording thermometers. Thermocouple measurement. Chemical indicators, autoclave tape. Biological control. |
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(vi) | Practicals | Safety measures, sterilization. Disinfection, waste disposal, check sterility. |
Topic | Sub-topic | T | P | |||
1. | INTRODUCTION TO MICRO-BIOLOGY | – Definition. – Nomenclature. – Classification. – Characterization. |
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2. | SAFETY | – W.H.O. code of practice. – Laboratory acquired infections. – Classes of laboratories. – Hazard groups. – Laboratory wastes. – Types of safety cabinets. – Handling and storage of chemicals. |
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3. | STERILIZATION | – Definitions. – Methods of sterilization. – Factors influencing sterilization. – Sterility testing. |
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4. | COLLECTION AND PROCESSING OF SPECIMENS |
– Specimen containers. – Types of specimens. – Transportation. – Processing. – Preservation. |
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5. | STAINING | – Preparation of a smear. – Types of stains. – Preparation of stains. – Staining methods. – Factors affecting staining. |
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6. | CULTURE MEDIA | – Types. – Ingredients. – Classes. – Preparation. – Storage. – Quality control. |
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7. | CULTURIVATION OF MICRO-ORGANISMS |
– Culture methods. – Factors affecting growth. – Cultural characteristics. |
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8. | IDENTIFICATION OF BACTERIA | – Biochemical tests. – Serological tests. |
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9. | ANTIMICROBIAL SUSCEPTIBILITY TESTING |
– Definition. – Mechanisms of action. – Factors affecting susceptibility tests. – Susceptibility testing. |
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10. | BACTERIOLOGY | Genus: – Staphylococcus. – Streptococcus. – Neisseria. – Escherichia. – Klebsiella. – Citrobacter. – Enterobacter. Yersinia. Salmonella. Shigella. Proteus. Haemophilus. Peudomonas. Vibrio. Brucella. Bordetella. Bacillus. Costridium. |
☐ Project title selections. | |||
☐ Sources of data | |||
☐ Available materials and equipment. | |||
☐ Finance/budget. |
Topic | Sub-topic | |||
1. | SAFETY | Safety techniques in microbiology laboratory. | ||
2. | STERILIZATION | Sterilization methods. Disinfection. | ||
3. | SPECIMENS | Collection. Transportation. Preservation. Processing. | ||
4. | LABORATORY DIAGNOSIS | Laboratory procedures used in diagnosis of common diseases. Sensitivity tests. |
1. | BACTERIOLOGY (CONTD.) | Corynebacterium. Mycobacterium. Borrelia. Leptospira. |
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2. | BACTERIOLOGY OF W ATER, MILK AND FOOD | Water sampling. Bacteriological analysis of water, milk and food. |
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Topic | Sub-topic | ||||
3. | MYCOLOGY | Definition. Morphological classification. Laboratory diagnosis. Diseases. Culture methods. |
Topic | Sub-topic | Content | |||
TERM II | Introduction General Chemistry |
Physical chemistry | Definition, diagnosis, importance. Atoms, atomic structure, valency, thermo-chemistry, redox, reactions, acids, bases, colligative properties, rates of reactions, theories of catalyses. |
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Inorganic reactions | Colour of solids and solutions, solubility, thermodynamics, qualitative analysis. | ||||
Volumetric analysis, titrimetric analysis is preferred |
Standard solutions, weights and measures, theory and choice of indicators, dissociation constants, buffers, pH, acid-base, redox and precipitation. | ||||
Titrations. | |||||
Organic chemistry | Structure of carbon compounds isomerism, homologous series, aromatic compounds, functional groups, reaction process. | ||||
Concepts of clinical chemistry |
Biochemistry | Amino acids and proteins, carbohydrates, lipids, vitamins, enzymes, nucleo-proteins, nucleic acids, porphyrins and bile pigments. | |||
Physiology organs | Kidney, liver, pancreas, stomach, lungs, heart. | ||||
Hormones | Origin, structure, general functions, control, feedback and other regulatory factors. | ||||
Blood fluids | Blood, ascitic, lymph, CSF. | ||||
Pathology | Nephrosis, renal calculi, diuresis, acidosis, alkalosis, hepatomegaly, cirrhosis, hepatoma, hepatitis, gallstones, myocardial infarction glycaemia, cancer of the pancreas, diabetes, gastritis, fertility hormones, thyroid hormones, tertiary hypercalcaemia. | ||||
Function tests | Renal, thyroid, liver cardiac, pancreatic, lipid profile. | ||||
Safety measures | Chemicals | Sources of injuries – carcinogenic, poisons, corrosives, volatiles, radio-active, explosives, fumes. Protective measures – protective gear, handling fire, fighting, gadgets and disposal. |
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Biological specimen | Sources of infection – exudates, stool aerosols, CSF. Protective measures – mechanical, electric thermal (hot water), air dry heat. Protective measures – protective gear, bench organization, insulation, voltage. |
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Laboratory ware | Sources of injuries – breakages, sharps, mechanical. Protective measures – protective gear, handling, disposal. |
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Specimen collection | Mode of collection Types of specimen |
Containers, anticoagulants. Disposable syringes and needles, labels, preservatives, request form, interpretation. Aseptic techniques, hygienic sites and stasis. Blood, urine, stool, CSF, aspirates, exudates. |
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Principle of techniques |
Pipeting | Capillarity, negative pressure, atmospheric pressure. | |||
Qualitative analysis | Physical examination, chemical analysis, chromatography, microscopy. | ||||
Quantitative analysis | Photometry, volumetric analysis, gravimetry, fluorimetry, electrochemistry chromatography, electrophoresis, radio-activity, automation. |
Diagnostic Techniques | Urine | Quantitive:Volume, colour, appearance, odour, sugar, ketones, bilirubin, urobilinogen, urobilin, urinary proteins, pH, crystals, casts, cells, SG, surface tension, nitrate, hormones, porphyrines. Quantitive: clearance, osmolarity, electrolytes, phosphates, enzymes, proteins, Glucose, hormones, porphyrins, electrophoresis, chromatography. |
Blood (plasma) | Qualitative:- Haemolysis, jaundice, coagulum, lipaemia. Quantitative: Sugars, proteins, urea, bilirubin, creatinine, electrolytes, uric acid, enzymes, hormones, lipids, HB AIC, inorganic phosphates, TIBC, electrophoresis, chromatography. |
Year 3 | Body Fluids | Qualitative: Appearance, coagulum, turbidity, volume, chemical analysis, pH. |
Term 2 | Quantitative: Biochemistry – proteins and sugars, titration, pH. | |
Resource Management | Fiscal | Financial Resources: Maintenance cost, record keeping, centralised Storage. |
Human | Personnel management, intersectoral collaboration. | |
Clinical | Nosographical | Epidemiology, sensitivity, correlation to reference ranges (values). |
Chemistry Research | Situation Analysis | Therapeutic drug monitoring (TDM) |
Year 3 | Project revision | |
Term 3 | Examination |
Topic | Sub-topic | |||
1. | INTRODUCTION | Definition. Importance. Safety precaution. |
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2. | BLOOD COMPOSITION | Erythrocytes. Leucocytes. Thrombocytes. Plasma. |
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3. | FUNCTION OF COMPONENTS | Erythrocytes. Neutrophilis. Monocytes. Lymphocytes. Eosinophils. Basophils. Thrombocytes. Plasma. |
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4. | HAEMOPOERISIS | Intra-uterine (foetal). Post-uterine (after birth). Extra-medullary hemopoeisis. (Myeloid metaplasia). Life-span of blood cells. |
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5. | HAEMATOLOGICAL SAMPLES | Anticoagulants. blood collection containers. Capillary blood sample. Venous blood sample techniques. |
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6. | PERIPHERAL BLOOD | Thin film preparation. Thick film preparation. Uses, purpose techniques. |
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7. | CYTOMORPHOLOGY OF BLOOD CELLS | Normal blood cells— (a) erythrocytes leucocytes platelets; (b) immature blood cells characteristics and significance. |
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8. | HAEMATOLOGICAL STAINS | Romanowsky stain: (i) Leishman. (ii) Jenners stains. (iii) Wrights stain. (iv) May grunwald. (v) Giemsa. |
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9. | HAEMOCYTOMETRY | Total blood cell counts. Red blood cells. White blood cells. Platelets. Differential leukocyte count. Battlement method. Longitudinal. Reticulocyte count. |
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10. | PACKED CELL VOLUME | Electronic blood cell counter. Micro haematocrit method. Macro haematocrit method. Electronic method. Sources of error techniques. |
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11. | ERYROCYTE SEDIMENTATION RATE | Wintrobe method. W estergren method. Laudau Adam’s. Electronic method. Factors affecting E.S.R. estimation techniques. |
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12. | HAEMOGLOBIN | Definition. Synthesis. Types of haemoglobin. Heamoglobinopathes. Methods of estimation. Haemoglobin curve. |
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13. | HAEMATOLOGICAL INDICES | Mean cell volume. Mean cell haemoglobin. Concentration. Mean cell haemoglobin (manual and electronic) techniques. |
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14. | SYSTEMATIC REPORTING OF THE FILM | Red blood cells. White blood cells. Platelets. SI blood parasites. Comments. |
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15. | ANAEMIA | Definition. Pathogenesis. Causes of anaemia. Classification. Types of anaemia. Laboratory investigation. Management. |
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16. | HAEMASTOSIS | Definition. Vascular system. Coagulation mechanism— (i) intrinsic mechanism, (ii) extrinsic mechanism, (iii) common pathway. |
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17. | FIBRINOLYSIS | *Investigation of haemostatic Bleeding time. Clotting time. Prothrombin time test. APTT Thrombin time substitution test. |
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18. | INTRODUCTION TO AUTOMATED COAGULATION, INTRODUCTION TO SUBSTITUTION TESTS |
Electronic methods. KCCT Substitution test. Using normal plasma. Using old serum. Using absorbed plasma. PTT substitution test. Using Russel viper venom. Thrombin time substitution test. Using reptilase. Using protamine sulphate. Using aminocarproic acid. |
Topic | Sub-topic | ||
Anaemia | – Definition. – Classification. – Clauses. – Types of anaemia. – Laboratory investigations. – Management. |
Topic | Sub-topic | ||
Haemostasis | – Definition. – Vascular system. – Coagulation mechanisms. (i) Intensive mechanisms. (ii) Extrinsic mechanisms. (iii) Common pathway. (iv) Fibrinolytic mechanisms. – Investigation of hemostasis (i) Bleeding time. (ii) Clotting time. (iii) Prothrombin time test. (iv) A.P.T.T. (v) Thrombin time. |
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Introduction to substitution tests | KCCT substitution test- (i) Using normal plasma. (ii) Using old serum. (iii) Using adsorbed plasma. P.T.T. substitution test— (i) Using russell viper venom. (ii) Thrombin time. Substitution test— (i) Using reptilase. (ii) Using protamine sulphate. (iii) Using amino caproic acid. Revision. |
1. | COLLECTION OF BLOOD | – Thin film. – Thick film. |
2. | PREPARATION OF ROMANOW SKY STAINS | – Leishman’s stains. – Field stain. – Leishman stain. – Marygrunwald stain. – Jenner’s stain. – Wrights stain. |
3. | ROMANOW SKY STAINING TECHNIQUES | |
4. | BLOOD CELL COUNT | – Total. – Differential. |
5. | SUPRAVITAL STAINS | – B.C.B. – New Methylene Blue. – Methyl Violet. |
6. | SUPRAVITAL STAINING | – Recticulocyte count. – Heinz body preparation. |
7. | PCV ESTIMATION | – Micro method. – Macro method. |
8. | E.S.R. | – Wintrobe’s method. – W estergren’s method. |
9. | HAEMOGLOBIN ESTIMATION | – Sahli method. – Cyanmethaemoglobin. – Oxyhaemoglobin method. – Haemoglobin curve. |
10. | REPORTING OF BLOOD FILM | – Red cells. – White cells. – Platelets. – Comments. |
11. | HAEMATOLOGICAL INDICES | – M.C.V. – M.C.H. – M.C.H.C. |
12. | BONE MARROW FILM PREPARATION | – Straight film. – Squash film. |
13. | IRON STAINING TECHNIQUE (PPB) | |
14. | L.E. BODY PREPARATION | |
15. | OSMOTIC FRAGILITY TEST | |
16. | HAEMOGLOBIN ELECTROPHORESIS (LAPE) |
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17. | HAEMOGLOBIN-F ESTIMATION (SINGER’S METHOD) |
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18. | G.G.P.D. SCREENING TEST | – Ivy’s method. |
19. | BLEEDING TIME TEST | – Duke’s method. |
20. | CLOTTING TIME TEST | – Lee and White Method. |
21. | PROTHROMBIN TIME TEST | |
22. | THROMBIN TIME TEST | |
23. | KAOLIN AND CEPHALIN CLOTTING TIME TEST (KCCT) OR A.P.T.T. | |
24. | WASHING OF GLASSWARE |
Topic | Sub-topic | ||
Introduction | Definition of the term. Blood transfusion service. Blood importance. |
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Terminologies | Antigen. Antibodies. Agglutination. Haemolysis. Sensitization. Precipitation. Complement. Hapten. Inhibition. Neutralization. Immunization. |
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ABO blood group system | History. Significance of ABO system. Inheritance. Antigens. Antibodies. ABO sub-groups. ABO grouping techniques. |
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Rhesus blood group system | History. Significance of Rh System. Inheritance. Antigens. Nomenclature. Variants of D antigens. Rhesus null phenotype. Rhesus grouping techiniques. |
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TOPIC (4) | |||
ABH blood group system | Definition. H-Gene. A-Gene. B-Gene. O-gene. Bombay phenotype. |
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Blood group specific substances | Definition. Types. Secretor status (Se gene). Significance. Techniques. |
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Other blood group systems | MNSS. Kell. Duffy. I. P. Lewis. Lutheran. Kidd. Xg. |
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Blood group anomalies | Conditional, physical. Hereditary. |
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Preparation of reagents used in blood transfusion | Normal saline. 22% bovine albumin. Coombs reagents (AHG). Lectins. Antisera. Enzymes. |
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TOPIC (5) | |||
Blood transfusion techniques | Direct coomb’s. Indirect coomb’s. Antibody screening test. |
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Antibody titration. Antibody identification. Absorption techniques. Absorption techniques. Elution techniques. |
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Crossmatching | Definition. Importance. Types. Phases. Techniques. |
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Transfusion reaction | Definition. Categories. Laboratory investigation. |
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Haemolytic disease of the new-borns | Definition. Causes. Clinical signs & symptoms. Laboratory investigation. Prevention. Management. |
Topic | Sub-topic | ||
Year 2 | Practical attachment (i.e. Term 5, 6, 7) -Perform tests listed in the practical rotation guideline. -Booklet. -Collection of data for project work. |
Topic | Sub-topic | ||
COMPLEMENT | INTRODUCTION TO COMPLEMENT SYSTEM. | ||
BLOOD DONOR SERVICE | Organization of blood donor centre. Blood campaigns. Recruitment – donors. Phlebotony procedure. Anticoagulants. Screening of donated blood. Documentation. Storage of blood. Disposal of blood. Quality control measures. Safety in blood bank. Maintenance of blood bank equipments. |
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BLOOD PRODUCTS | Definition. Types. Uses. Preparation. Storage. |
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APPLICATION OF BLOOD GROUP TO FORENSIC MEDICINE | 1st order of exclusion. 2nd order of exclusion. Differentiation of human stains from others. |
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NATIONAL POLICY GUIDELINES IN BLOOD TRANSFUSION | Collection of blood. Distribution. Uses. Legal aspects. |
Topic | Practicals | ||
ABO blood group system | Slide and tube. Forward and reverse grouping. ABO sub-group. |
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Rhesus blood group system | Slide method. Tube method. 22% bovine method. |
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Coomb technique | Direct Coomb’s test. Indirect Coomb’s test. Du test. Anti-body screening test. |
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Neutralization test | Neutralize natural antibodies. Detection of blood group specific substance. |
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Enzyme technique | Low’s method (papain method). | ||
Anomalies | Para-agglutination. Chmeras. Blood group A with anti A in serum. Blood group O from an infant. |
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Blood donor centre | Blood campaign. Blood donor recruitment. Maintenance of record. Phlebotomy. Blood screening for— HIV, Hepatitis, VDRL, Mass blood grouping. Preparation of various blood products— packed cells, white cell concentrate, poor white cell plasma, platelet rich plasma, FFP – fresh frozen plasma, etc. |
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Preparation of reagents | Physiological saline. Preparation of 22% bovine albumin from 30%. |
Topic | Sub-topic | ||
Introduction to medical parasitology and medical entomology | – Definition and terminology. – Classification. – Host-parasite relationship. – Modes of transmission. – Harmful effects of parasite on hosts. – Safety precautions. – Collection/reception of specimens. – Preservation. – Transportation. – Storage. – Preparation of reagents and stains. – Epidemiology and surveillance of parasitic infections. – Equipment and apparatus. – Quality assurance. |
||
Parasitological and Entomological techniques | – Direct wet preparations. – Concentration methods. – Smears swabs and cultures. – Xenodiagnosis. – Immunodiagnosis. – Collection of arthropods. – Mounting and labelling. – Identification. – Dissections. – Insectary. |
||
Helminthology | – Introduction and terminologies. – Classification. – Geographical distribution. – Lifecycles. – Morphology of developmental stages. – Pathogenesis and pathology. – Laboratory diagnosis. – Epidemiology, prevention and control of helminthic infections. |
||
Medical Entomology | – Introduction and terminologies. – Classification. – Lifecycles. – Morphology of developmental stages. – Identification of vectors. – Medical importance. – Geographical distribution. – Prevention and control of vectors. |
||
Malacology | – Introduction and terminologies. – Classification. – Lifecycles. – Morphology and identification of vector snails. – Geographical distribution. – Medical importance. – Control. – Malacological techniques. – Collection and transportation of molluscs. – Carcarial shedding and identification. – Preservation and identification of molluscan shells. |
||
Protozoology | – Introduction and terminologies. – Classification. – Geographical distribution. – Lifecycles. – Morphology of developmental pathogenesis and pathology. – Laboratory diagnosis. – Epidemiology, prevention and control of protozoan infections. |
||
Mammalogy | – Introduction and terminologies. – General classification of animals. – Animal house. – Management, use and disposal of laboratory animals. – Medical importance. – Control of mammalian reservoir host. |
||
Insectcides and molluscides | – Classification. – Formulation. – Introduction to application methods. – Safe use. |
||
MEDICAL PARASITOLOGY | PRACTICALS. | ||
Introduction | – Receiving, recording and storing specimens. – Care and use of laboratory equipment and apparatus. – Educational visit to meteorological station. |
||
Parasitological techniques | – Direct wet preparations. – Concentration methods— sedimentation. modified formal ether. zinc sulphate floatation. membrane filtration. brine floatation. – Parasite count— Kato thick smear. Stoll’s method. MacMaster chamber. Malaria/QBC. – Swabs and smears. – Cultures. Immunodiagnosis. |
||
Protozoology | – Collection, processing and examination. – Identification of diagnostic stages. – Reporting the findings. – Analysing the results. |
||
Helminthology | – Collection, processing and examination of specimens. – Identification of diagnostic stages. – Reporting the findings. – Analyzing the results. |
||
Medical entomology | – Collection and mounting of anthropods. – Identification and labelling. – Preservation and storage. – Dissections. – Xenodiagnosis. – Educational visit to insectary. |
||
Malacological techniques | – Collection and transportation of molluscs. – Carcarial shedding and identification. – Preservation and identification of molluscan shells. |
||
Mammalogy | – Management and use of laboratory animals. – Disposal of laboratory animals. – Destruction. – Destruction of reservoir hosts. |
||
Insectcides | Preparation for use— insectcides/imagicides. larvicides. molluscides. acaricides. miticides. tungicides. – Storage and disposal of chemicals. |
Year | Term | Topic | Sub-topic |
ONE | TWO | INTRODUCTION | – Definitions: Viruses. Virology |
– General properties of viruses. – Classification of viruses criteria. |
|||
EPIDIMEOLOGY OF VIRAL DISEASES | – Acute infections. – Chronic infection. – Slow infections route of spreading of viruses to the community. |
||
BIOSAFETY | – Categorisation of pathogens to risk groups. – Activities harmful to the worker and others in virology. – Occurrence of laboratory infections and their prevention. – Location of health and safety equipment in the work place e.g. fire extinguisher. – First-aid kit. – Use of safety gear. – Use of pipetting aids. – Use of safety cabinets. |
||
THREE | |||
PATHOGENESIS | Clinical and sub-clinical and latent – infections. – Virulence. – Localized and systemic infections. |
||
USE OF EQUIPMENT | Use and care of equipment and instruments in a virus laboratory. Inverted microscope. Water baths. Refrigerated centrifuge. Deep freezer. Refrigerator. Autoclave. Incubator CO2. Cool boxes. Elisa equipment. Biosafety cabinets. |
||
VIROLOGICAL SPECIMENS | Types of specimen collection. Specimen containers. |
||
VIROLOGICAL | Handling. Transportation. Storage. Preservation. |
||
SYSTEMATIC VIROLOGY | RNA viruses. Unclassified viruses. RNA virus. (Introduction). |
Topic | Sub-topic | ||
SAFETY | – Bio-safety technology in virology laboratory. | ||
STERILIZATION | – Sterilization techniques, disinfection. | ||
EQUIPMENT | – Care of virology equipment. | ||
SPECIMENS | – Collection containers, transport, storage, preservation. | ||
LABORATORY DIAGOSIS | – Laboratory procedures used in diagnosis of common diseases. | ||
ANIMAL HOUSE | – Handling, bleeding, injection, feeding. |
Term 8 |
Systemic specimen processing and laboratory diagnosis |
DNA viruses processing Tissue culture Animal inoculation Serology/immunological techniques |
T P T/P |
13 Hrs 10 Hrs 22 |
9 | Laboratory diagnosis Treatment and vaccination of viral diseases Total quality management |
Elisa RPHA Simple rapid assyas Target sites for antiviral drugs Introduction to vaccinations Definition and concepts TQM QC and QA Designs and benefits Project |
T/P T/P T/P T T T |
22 18 7 4 2 |
Year | Topic | Sub-topic | ||||
Introduction to immunology | – Definition of immunology. – History of immunology. – Development of vaccines e.g. vaccines. |
|||||
Adaptive and innate immunity | Immune system. Adaptive immune system— -natural. -artificial. |
Biology of immune system | – Primary lymphoid organs. – Secondary lymphoid organs. – Dissection of a named laboratory animal e.g. mouse, rat, or guinea pig to display the primary and secondary lymphoid organs. |
|||
Cell involved in the immune system | B Lymphocytes. T Lymphocytes. The mononuclear phagocyte system. Polymorphonuclear granulocytes. Thin blood smear. Staining techniques. Identification of cells. |
|||||
The major | – Arangements of MHC genes. |
|||||
Histocompatibility | – Functions of MHC antigens Class I, II, III. |
|||||
Immunochemistry | – Immunoglobulins— -structure. -classification. - distribution. – Antibody – antigens reactions. – The agglutination reaction. – Precipitation tests. – Haemolytic immune. – Body titration. – Compliment system. – Complement titration. – Theories of antibody formation. |
|||||
Antigen recognition and cell co-operation in immune responses |
B-cell antigen recognition. T-cell antigen recognition. Antigen presenting cells— – primary immune response, – secondary immune responses, – immunological memory. |
|||||
Hypersensitivity states | Type I. Type II. Type III. Type IV. Demonstrate type O Reaction using guinea pig. |
THREE | ||
Auto-immunity | – Self tolerance. – Emergence of auto-immune disorders. |
|
Transplantation and rejection | – Tissue transplantations. – Organ transplantations. |
|
Immuno-deficiency | – Definition. | |
States | – Primary immuno-deficiency. – Secondary immuno- deficiency. – Mechanisms leading to immuno-deficiency. – Methods of investigation. – Elisa. |
|
Infection and immunity | – Mechanisms of immunity to infectious diseases. – Antibody antigen reactions. |
|
– Agglutination. – Precipitation. – Haemagglutination. – CFT. – Elisa. |
Topic | Sub-topic | ||
SAFETY LABORATORY ANIMALS | Biosafety techniques in an immunology laboratory. Handling. Bleeding. Injection. |
||
IMMUNO-CHEMISTRY | Antibody separation. | ||
IMMUNOLOGICAL TECHNIQUES | Ab-Ag reactions. Agglution. Precipitation. CFT. Elisa. ETC. |
||
EQUIPMENTS | Use and care of the equipment. |
_________________________
APPENDIX 1
TRAINING STANDARDS
STAFF/STUDENT RATIO:1.LECTURERS:THEORY: 1:0.PRACTICAL: 1:5.2.SUPPORT STAFF:TECHNOLOGIST (DIPLOMA LEVEL) ONE (1).TECHNICIANS TWO (2).3.ACADEMIC STAFF QUALIFICATIONS:Minimum HD MLS with three (3) years experience plus a certificate inMedical EducationORHD MLS with (5) years working experience and good track record.4.ATTENDANCE – 90%.5.DURATION OF PROGRAMME – Three (3) years.6.DISTRIBUTION OF LEARNING:THEORY – 50%.PRACTICAL – 50%.7.SUBJECTS TAKEN: ALL.8.AVERAGE PASS MARK – 50%.9.EXAMINATION DECLARATION—Common examination shall be given to all students.Examination results shall be declared two weeks after the last paper.______________________________
APPENDIX 2
ESSENTIAL EQUIPMENT
MICROBIOLOGY
1. Autoclave (portable) | 1 between 10 students | ||
2. Medium water bath | 1 between 5 students | ||
3. Lovibond comparators – assorted | |||
4. pH meters | 1 between 5 students | ||
5. Anaerobic jars | 1 between 5 students | ||
6. Incubators/hot air oven (adjustable) | 1 between 10 students | ||
7. Distillers | 2 for the whole institute | ||
8. De-ionizers | 2 (small) | ||
9. Microscopes binocular | 1 between 10 students | ||
10. Weighing balance | 1 top pan load balance | ||
11. Woods lamp | 1 | ||
12. Centrifuge | 1 between 4 students | ||
13. Bunsen burner/spirit | 1 between 2 students | ||
14. Tripod stands/asbestos mat | 1 between 10 students | ||
15. Fridge/deep freezer | 1 between 10 students | ||
16. Safety cabinet | 1 per laboratory | ||
17. Teaching microscopes | 1 between 10 students | ||
18. Mechanical shaker | 1 between 10 students | ||
19. Inoculating loops | 1 per student | ||
20. Assorted microbiology glassware | adequate | ||
CLINICAL CHEMISTRY | |||
1. Colorimeters | 1 between 4 students | ||
2. Analytical balance – top pan loading | |||
3. Sensitivity up to 1 mg | 1 between 5 students | ||
4. Flame photometers | 1 between 10 students | ||
5. Centrifuge | 1 between 4 students | ||
6. Refrigerators/freezers | 1 between 10 students | ||
7. Water bath medium | 1 between 4 students | ||
8. pH meter | 1 between 5 students | ||
9. Mechanical mixers | 2 | ||
10. Electrophoresis equipment | 2 per institution/class | ||
11. Distiller/de-ionizer | 2 | ||
12. Hot air oven/(incubator) adjustable | 10 | ||
13. Flame photometer | 1 between 5 students | ||
14. Ion selective electrodes | 2 of item | ||
15. Electrophoresis equipment | 1 between 10 | ||
HAEMATOLOGY | |||
1. Haemoglobinometers | 1 | ||
2. Centrifuge | –ditto– | ||
3. Microhaematocrit centrifuge | 1 between 5 students | ||
4. Microscopes – Blood mixers rollers | 1 between 10 students | ||
5. Water bath | –ditto– | ||
6. Incubator | –ditto– | ||
7. Colorimeter | –ditto– | ||
8. Electrophoresis equipment | 1 between 10 students | ||
9. Sphygmomanometer | 1 between 5 students | ||
10. E.S.R. stands | 1 between 4 students | ||
11. Deep freezer/fridge | 1 between 10 students | ||
12. Deep freezer | 1 between 5 students | ||
13. Coulter counter | 1 for each class | ||
14. Neubaeur chambers | 1 each student | ||
15. Distiller | 2 per institution/class | ||
16. Analytical balance | 1 between 10 students | ||
17. Stethoscopes | 1 between 5 students | ||
18. Spectroscope – direct vision/revision | |||
19. Refrigerated centrifuge | 1 | ||
HISTOPATHOLOGY | |||
1. Microtome rocking/rotary | 1 per 4 students | ||
2. Manual tissue processing set | 1 between 4 students | ||
3. Hot plate | 1 between 6 students | ||
4. Hone and strope | 1 between 4 students | ||
5. Automatic knife sharpener | 1 per class/institution | ||
6. Water bath, medium size | 1 between 4 students | ||
7. Microscope (teaching) | 1 for the institution | ||
8. Cold plate | 1 between 6 students | ||
9. Weighing balances | 1 between 5 students | ||
10. De-ionizers | 1 per class/institution | ||
11. Fume chambers | 1 per laboratory/institution | ||
12. Automatic tissue processor | 1 per class/institution | ||
13. Automatic staining machine | 1 per class/institution | ||
14. Freezing microtone - hard set | 1 per class/institution | ||
15. Centrifuge | 1 per class/institution | ||
BLOOD TRANSFUSION SCIENCE | |||
1. Blood bank refrigerator | 1 per class/institution | ||
2. Grouping tiles | 1 per student | ||
3. Water bath | adjustable (medium size) | ||
4. Plasma extractors | 15 students | ||
5. Centrifuges | 1 between 4 students | ||
6. Weighing balance | 1 between 5 students | ||
7. Syphomomanometers | 1 between 5 students | ||
8. Hot air oven (adjustable) | 1 in the institution | ||
9. De-ionizers and stillers | 1 per class/institution | ||
10. Mechanical shaker | |||
11. Blood transfusion bleeding unit | |||
12. Assorted blood transfusion glassware and adequate apparatus. | |||
13. Microscopes | 1 per 2 students | ||
14. Deep freezer 70 degrees centigrade | |||
15. Automated centrifuge for blood products | |||
16. Cool boxes. | |||
MEDICAL PARASITOLOGY | |||
1. Microscopes | 1 for 4 students | ||
2. Centrifuge | –ditto– | ||
3. Refrigerators | –ditto– | ||
4. Pestle and motor | 1 per student | ||
5. Teaching microscope | |||
6. QBC unit | |||
7. Assorted apparatus e.g. sieves racks, test- tubes, stirring rods, applicator sticks, forceps funnels, Kato kits, hand lenses. |
|||
8. Stereo microscope/dissecting microscope. | |||
9. Fluorescent microscope | 1 per class | ||
10. Geiger Muller counter/scintillator |
VIROLOGY
1.Hepatitis Screening equipment.2.H.I.V. Screening equipment—(a)Eliza(b)Immunoblots (W estern Blot)(c)P.C.R. (Polymerase chain reaction)3.CD4/CD8 Counting machine4.Viral load machine5.Tissue lines6.Immuno fluorescent equipment7.Inverted microscopesIMMUNOLOGY
1.Mechanical shakers2.Centrifuges3.W ater baths4.Refrigerators5.Geiger Muller counter6.Chromatographic sets—(a)G.L.C. gas liquid chromatography(b)H.P.L.C. high pressure liquid chromatography(c)T.L.C. thin layer chromatography7.Thermocycler.__________________________
APPENDIX 3
ESSENTIAL BOOKS
Title | Author | |||
1. | Introduction of medical laboratory technology | F.J. Baker et al | ||
2. | Medical Laboratory Manual for Tropical Countries Part I and II |
Monicah Chesbourough |
MEDICAL MICROBIOLOGY
Title | Author | |||
1. | Review of Medical Microbiology | Ernest Jawetz, et al | ||
2. | Medical Microbiology. A guide to Microbial Infection, Pathogenesis, Immunity and Laboratory Diagnosis and Control |
David Green | ||
3. | Clinical Bacteriology | Joan E. Stocks | ||
4. | Bacteriology Illustrated | Giels and Dodd | ||
5. | Practical Medical Microbiology | J.G. Colle et al | ||
6. | Hand book of Bacteriology | Baker et al |
CLINICAL CHEMISTRY
Title | Author | |||
1. | Practical Clinical Chemistry Vol. I & II | Harold Varley |
Title | Author | |||
2. | A Basic Biochemiostry | Hayashi et al | ||
3. | Essentials of Volumetric Analysis | Lambert | ||
4. | Biochemistry a Case Oriented Approach | Montgomery |
HAEMATOLOGY
Title | Author | |||
1. | Practical Haematology | Dacie and Lewis | ||
2. | Clinical Haematology in Medical Practice | De Gruchy | ||
3. | Essential Haematology | Petit | ||
4. | Atlas of Haematology | Macdonald Dodds |
HISTOPATHOLOGY
Title | Author | |||
1. | Carleton’s Histological Techniques | Drory and W ellington | ||
2. | Theory and Practice of Histological Techniques | Bancroft | ||
3. | Cellular Pathology Technique | C.F.A Culling et al | ||
4. | Text/Atlas of Histology | Leeson and Pagaro | ||
5. | Basic Histology | Luis Carlos et al | ||
6. | Practical Section Cutting and Staining | Clayton |
BLOOD TRANSFUSION
Title | Author | |||
1. | Blood Serology | Boorman and Dood (England Edition) | ||
2. | Modern Blood Banking and Transfusion Services |
F. Harmening & Pittiglio | ||
3. | Blood Trasfusion Guidelines | Ministry of Health | ||
4. | Blood Groups in Man | Race and Sanger | ||
5. | Blood Transfusion in Clinical Medicine | Mollison Patrick | ||
6. | Blood Group Technique | S.I.B. Harris | ||
7. | Technique in Blood Grouping | Ivon Danford et al |
MEDICAL PARASITOLOGY
Title | Author | |||
1. | Basic Clinical Parasitology | Harold W . Brown | ||
2. | Introduction to Parasitology | A.C. Chandler | ||
3. | Worms and Diseases | R. Muller | ||
4. | Tropical Diseases | R. Muller | ||
5. | Medical Entomology | Patton W .S. | ||
6. | Parasitic Disease in Man | Richard Knight | ||
7. | Lecture Notes on Entomology | M.W . Service | ||
8. | Atlas of Medical Helminthology Proto-zoology | Jeffrey and Leach |
VIROLOGY
Title | Author | |||
1. | Practical Virology for Medical Students and Practitioners |
D. Metasalaar et al | ||
2. | Fundamentals of Medical Virology | Kucera and Louis S. | ||
3. | Virological Procedures | Hopkins et al | ||
4. | Virology – Practical Approach | B.S. Nahy et al | ||
5. | Medical Virology | D. W hite & F. Fenner | ||
6. | Medical Virology – A Practical Approach | Editor – U. Desselberger | ||
7. | Principles of Molecular Virology | A.J. Cann |
IMMUNOLOGY
Title | Author | |||
1. | The Principles of Immunology | Ivan Roitt | ||
2. | Fundamentals of Immunology | Tesdale | ||
3. | Practical Immunology | Hudsons and Hay | ||
4. | Practical Immunology | Talwar | ||
5. | Basic & Clinical Immunology | Peakman & Vergains | ||
6. | Understanding Immunology | Peter Woods & Prentice-Hall |
THIRD SCHEDULE [r. 2]
HIGHER DIPLOMA CURRICULUM
MINISTRY OF HEALTH | ||
THE KENYA MEDICAL LABORATORY | ||
TECHNICIANS AND TECHNOLOGISTS BOARD | ||
CURRICULUM FOR HIGHER DIPLOMA IN MEDICAL LABORATORY SCIENCES | ||
TABLE OF CONTENTS |
___________________________________
COURSE TITLE: "HIGHER DIPLOMA IN MEDICAL LABORATORY SCIENCES"INTRODUCTIONThe aim of this course is to produce is to produce specialists in various Disciplines of Medical Laboratory Sciences.RATIONALEThere is inadequacy of personnel at specialist level in the Management of Medical Laboratory Services. Due to this there is not enough supervision in the maintenance of quality service in the various disciplines of Medical Laboratory Sciences. The training of Medical Laboratory Technology needs to be improved and updated regularly in order to cope with the dynamism in Medical Laboratory Sciences, hence the need for training at this level.The Higher Diploma holder should be trained well enough to carry out the following roles:—TERM | HRS ON CAMPUS | HRS IN CLINICAL PLACEMENT |
1 | 440 | — |
2 | 320 | 120 |
3 | 440 | — |
Total | 1 200 | 120 |
Theory Papers | – | 100 Marks |
Practicals | – | 3 hrs – 150 marks |
Oral Project | – | 50 marks |
Topic | Sub-topic | |||
1. | Process of developing a presentation |
Planning. Preparing. Presentation. |
||
2. | Presentation | Launching a presentation software. Setting-up a file. Creating slides. Animating a presentation. Retrieval of a presentation. Printing handouts and notes. Staging a presentation. |
||
3. | Principles of networking | Principles of networking. Types of networks. Benefits of networks. Data security in a network environment. |
||
4. | Internets | Introduction to internet. Websites. Results of internet searches. Printing, copying and saving internet. Searches. E-mail services. |
||
5. | Searching techniques | Defining the search topics. Use of search engines. Abbreviations and list of medically important journal. Relevant home pages. |
||
6. | Statistical analysis of data | Launching of SPSS. Defining variable. Setting up files. Transforming data. Computing analysis of data. Interpreting outputs. |
Topic | Sub-topic | Time | |||
Epidemiology | Definition, terms used. | ||||
Types | Descriptive. Analytical. |
||||
Use of epidemiological data | Planning for resources. Classification of diseases. Describing determinates of disease. Observation and experimental. |
||||
Study designs | Cross-sectional. Prospective. Retrospective. Experimental (Intervention). Clinical. |
||||
Disease patterns | Primary. Secondary. Tertiary. |
||||
Screening and surveillance | Types. Application. Uses. |
No. | Topic | Sub-topic | ||
1. | Overview of management | – Role of managers in organisations. – Process of management. – Importance of management in organisations. |
||
2. | Techniques of organising | – Process of planning. – Importance of planning. – Techniques used in planning. – Strategic planning. |
||
3. | Techniques of organising activity | – Basis of organising activities. – Circumstances of choosing basis. – Merits and demerits of each base. |
||
4. | Techniques of co-ordination | – Process of co-ordination. – Role of manager in directing organisational activities. – Techniques used in co-ordination. |
||
5. | Human resource co-ordination | – Structuring the system (analysing and designing jobs). – Recruitment, selection, placement internal mobility. – Separation, death, retirements or resignation. – Performance appraisal. – Preparing for advancement. |
||
6. | Management of change and conflict resolution |
– Factors influencing organisation changes. – Causes of resistance to change. – How to overcome employee resistance to change. – Techniques of managing change. |
||
7. | Techniques of managing time | – Meaning of conflicts. – Advantages and disadvantages of conflicts in an organisation. |
||
8. | Stress management | – Meaning of stress. – Causes of stress. – Techniques of managing stress. |
||
9. | Techniques of control in organizations |
– Importance and nature of control. – Types of control systems. – Control techniques. |
||
10. | Problem solving techniques | – Importance and nature control. – Stages of problem solving. – The merits and demerits of each. – Problems solving techniques. – Barriers to effective problem solving. |
||
11. | Stores management | – Inventory control systems. – Setting order quantities. – Stock records. |
||
12. | Public finance | – Sources of Government funds. – Public finance. – Government budgetary cycle. – Budgetary control analysis and interpretation. – Book-keeping. – Accounting for donor funds. – Small business enterprises. |
||
13. | Community development | – Community work. – Community organisation. – Community participation. – Community surveys diagnosis. – Intra- and inter-sectoral collaboration. – Field visits. |
||
14. | Quality management techniques | – Need for quality maintenance. – Strategies for quality maintenance. – Limitations of the strategies for quality management. – Measures for approving quality performance. |
Topic | Sub-topic | ||
---|---|---|---|
Introduction | Definition of research. Types of research: • pure research. • applied research. • action research. Justification of research. Ethical considerations. |
||
Methods of research | Descriptive research. Survey. Correlational. Retrospective. Experimental. Action research. |
||
Research design | Purpose. Hypotheses research, questions, objective, formulation. Characteristics of good research designs. Pre-experimental designs. True experimental designs. Quasi experimental designs. Export factor designs. |
||
Population and sampling | Review of— Population. Types of sampling. Probability sampling method. Non-probability sampling method. |
||
Data collection | Observation methods. Interviews and questionnaires. Trace measures. Content analysis. Data archives. Measurements. |
||
Data analysis | Qualitative method. Quantitative method. Review of— – Binomial distribution. – Poison distribution. – Normal distribution. – Student distribution. – ANOVA. – X2 test. – F –test. – Test of hypothesis in large and small samples. |
||
Use of computer | In statistical data analysis. In data organisation. Production report. |
||
Project write-up | Selection of project. Documentation of sources. Development of proposal. Carrying out of project. Reporting. Layout of reports. Data presentation. |
||
Project management | Needs assessment. Proposal preparations and presentations. Implementations. Monitoring and evaluation. Impact evaluation and sustainability. |
Topics | Sub-topics | |
1. | Social and development studies |
Medical psychology. Medical sociology. Economics. Social economics and development. Government of Kenya. National philosophy. Science and technology. Commerce. Public relations. Development theories. Natural environment and development. |
2. | Ethics | Revise meaning and importance. Major religions of the world and influence of religion on ethics. Professional ethics and conduct. With particular reference to Part VI of the Constitution of the Association of Kenya Medical Laboratory Scientific. |
Topics | Sub-topics | |
Officers and any other relevant codes of Ethics. Technology and religion. Natural law. |
||
3. | Law | Revise definition & importance. Sources of Kenyan Law. Constitution of Kenya, relevant Acts of Parliament and subsidiary legislation. Law of contract. Law of torts. Family law. Land law. |
4. | Guidance and counselling | Guidance. Counselling. Counselling techniques. |
Topic | Sub-topic | T | P |
History of microbiology | Major contributors. Development. Introduction of micro-organisms and disease. |
T | P |
Taxonomy | Classification. Nomenclature. Identification. |
T | P |
Safety | Laboratory associated infections. Precautions against accidents in laboratory. Safety cabinets. Disinfection and decontamination of laboratory wastes. Handling of chemicals and laboratory animals. |
T | P |
Sterilization | Definition. Methods of sterilisation. Factors affecting sterilisation. Quality control of sterilisation. |
T | P |
Microbial genetic and molecular microbiology |
Basis of heredity. Mutations. Gene transfer. Drug resistance. Molecular techniques. |
T | P |
Sources of transmission of microbial infections |
Sources. Transmission routes. Types of infections. |
T | P |
Pathogenicity | Association to the host. Pathogenic mechanisms. | ||
Specimens | Types. Containers. Methods of collections . Transportation. Processing. Preservation. |
T | P |
Staining | Preparation of smears. Types of stains. Preparation. Staining methods. Factors affecting staining. |
T | P |
Culture media | Types. Ingredients. Classes. Preparation. Storage. Quality control. |
T | P |
Cultivation of micro-organisms | Culture methods. Factors affecting growth. Cultural characteristics. |
T | P |
Identification of micro-organisms |
Biochemical tests. Serological tests. Phage typing. Colicine typing. P.C.R. Animal pathogenicity (inoculation). |
T | P |
Antimicrobial susceptibility testing | Definition. Mechanisms of action. Factors affecting susceptibility tests. Susceptibility testing. Drug assays. |
T | P |
Topic | Sub-topic | ||
Systemic bacteriology and mycology | Genus— Staphylococcus. Streptococcus. Neisseria. Branhamella. Veillonella. Escherichia. Citrobacter. Klebsiella. Proteus. Pseudomonas. Serratia. Providencia. Salmonella. Shigella. Morganella. Yersinia. Brucella. Gardnetella. Francisella. Bordetella. Alcaligenes. Pasteurella. Haemophilus. Corynebacrerium. Listeria. Erysipelothrix. Bacillus. Clostridium. Myeobacterium. |
||
Project | Data collection. Data analysis. |
Topic | Sub-topic | T | P |
Spirochaetes | Treponema. Borrelia. Leptospira. | ||
Rickettsiae | Rickettsiae. | T | P |
Chlamydia | Chlamydia. | T | P |
Mycology | Superficial Mycosis. | T | P |
Public health | W ater. Food. Milk. Air. |
T | P |
Quality control | Specimens. Microbial techniques. Culture media. Stains. Equipment. Report and record. |
T | |
Project | Data analysis. Project report write-up. Presentation. |
Topic | Sub-topic | Contents | |
Concept of Clinical Chemistry 220 hrs |
Review of general chemistry Physical chemistry |
Atoms, atomic structure, valency. Thermo chemistry, redox reactions, acids, bases, colligative properties, rates of reactions, theories of catalyses. |
T |
Inorganic chemistry | Colour solids and solutions. Solubility, thermodynamics. Qualitative analysis. | T/P | |
Volumetric analysis | Standard solutions, weights and measures, theory and choice of indicators, dissociation constraints, buffers, pH acid-base, redox and precipitation titration. | T/P | |
Organic chemistry | Structure of carbon compounds. Isomerism, homologons series, aromatic compounds, functional groups, reaction processes. |
Topic | Sub-topic | Content | |
Concept of clinical chemistry | Biochemistry Biologic oxidation | Redox reactions, phosphate bond energy, co-enzyme cytochrome C. | T |
Bio-molecules | Classification, structure. Metabolism, pathology of— Carbohydrates, lipids, amino acids and proteins nucleoproteins and genetic coding, vitamins, porphyrines, enzymes. |
T | |
Physiopathology | Liver, kidney, heart, gut, pancreas, lungs, endocrines, blood, CNS, inorganic constituents of the body fluids. | ||
Drugs & poisons | Therapeutics. Drugs of abuse. Poisons. |
T/P | |
Oncology | Tumour markers, biochemical effects of diffused endocrine. System, carcinoid and multiple. Endocrine adenopathy (MEA). |
T/P |
Topic | Sub-topic | Content | |
Foetal chemistry | Amniocetesis Bilirubin Neonatal thyroid functions. Plasma alpha feto proteins, Eustriol. |
T/P | |
Inborn error of metabolism | Genetics, metabolic pathways. Screening of inborn errors of metabolism, laboratory diagnosis, pathology. |
T/P | |
Total Quality Management (TQM) |
Quality assurance | Specimen collection. Quality assessment. Quality control. | |
Reference ranges | Factors affecting reference. Values. Population studies in deriving reference values, multivarial normality, diagnostic uses of reference values. |
T/P | |
Resources | Planning, procurement, fiscal, human, stock control. | ||
Principles of specialized instruments | Auto analyser | Central processing unit (CPU). Reagent control unit (RCU). Sample control unit. Data control unit. |
|
Scintillation counter | Radio Immuno-labelling. | T/P | |
Immuno-chemistry auto analyser | Micro-particle-enzyme-immuno-assay (MPEIA). Enzyme linked immuno-sorbent. Assay (ELISA). |
T/P | |
HPLC/GLC | Chromatographic separations. | ||
Electrophoresis systems |
Isoelectric focusing. Zone electrophoresis. SD-PAGE electrophorsis. High voltage elctrophorsis. |
T P | |
Blood gas analyser | Electrochemistry. | T/P | |
Thermocycler | Polymerase chain reactions. | T/P | |
Operations of specialized instruments | Random access systems | Initialization, programming, standardization, loading, data collection. |
Topic | Sub-topic | Contents | |
Principles of techniques | Photometry | Endpoints, kinetics, EIA, absorption, emission, tubdimetry. | T/P |
Separation | Chromatographic, electrophoresis, diffusion. | T/P | |
Electrochemistry | ISE, Ion Exchange Resin. | T/P | |
Fluorimetry | IFT, MEIA, ELFA. | T/P | |
Radiation | RIA. | T/P | |
Thermocycler | Polymerase chain reactions (Nucleoproteins: DNA, mRNA, TRNA, HLA typing). | T/P | |
Techniques | Function profiles | Malabsorption, RFT, LFT’S, lipid, collagen, gastric, muscle enzymes, cardiac enzymes, fertility hormones, triple tests, thyroid hormones, protein profile. Allergy and allergens, blood gases, Hb and derivatives, electrolytes. Corticosteroid hormones. |
T/P |
Specific measurements | C-peptide, HBAIC, GTT, insulin, 5H1AA, 17-ketostosteoids, VMA, catecholamines, G6 PD, 17 hydroxycorticosteroids, porphyrins, osmolarity, reducing substances, CEA, CA 125, CA 15.3, CA 1.9, PSA, AFP, B.HCG, microproteins, electrophoresis, HPLC, GLC Iron and TIBC, parathyroid hormone, thyroid antibody, cardiolipin antibody. |
T/P | |
Research | Nosographical Situation analysis |
Epidemiology, sensitivity. Correction to reference ranges. Therapeutic drug monitoring. Nutrition, physiotherapy. |
Topic | Sub-topic | |||
1. | Cytochemistry of haemopoiesis | RNA. DNA. Defective. Erythropoiesis. |
||
2. | Haematological reagents | Romanousky stain. Supravital stains. Cytochemical reagents. Anticoagulants. Other routine haematological reagents and storage. |
||
3. | Anaemia | Iron deficiency. Megaloblastic anaemia. Aplastic anaemia. Sideroblastic anaemia. Haemolytic anaemia. Haemoglobinopathies. |
Topic | Sub-topic | ||
Enzymopathy | G6PD. Pyruvate kinase. Analysis techniques. Peripheral blood film examination. |
||
Haemolytic screening procedures | Reticulocytes count. Heinz body preparation. |
||
Osmotic fragility test. Hb electrophoresis. Hb F estimation. Direct Coomb’s test. Antibody screening test. Bilirubin Estimation. Polypeptide assay. Ham’s test. Estimation of Hb A2. |
|||
Haemostasis | Vascular System. Blood coagulation. Fibrinolytic mechanism. |
||
Coagulation screening procedures | Haemogram. Prothrombin time test. Activated partial thromboplastin time test. Thrombin time test. Substitution tests. Euglobin clotlysis test. International normalized ratio. Bleeding time test. Clotting time test |
||
Blood coagulation factor assay with specific reference | Factor IX. Factor I. Factor II. Factor VII. Factor V. Factor XIII. |
||
Automatic coagulation procedures | Routine tests. Specialized techniques. |
||
Coagulation therapy with specific reference to | Heparin. W arfarin/koumarin. Monitoring. |
||
Leukaemia | Acute lympoblastic leukaemia. Acute myoloblastic leukaemia. Chronic lymphocytic leukaemia. Chronic granulocytic leukaemia. Chronic monocytic leukaemia. Acute yelomonocytic leukaemia. Erythroleukaemia. |
||
Leukaemia | Lymphoid leukemoid reaction. Myeloid reaction. |
||
Aleukaemoid. Psedoleukaemia. |
|||
Bone marrow specimen | Collection procedure. Processing procedure. Examination and reporting. |
||
Cytochemical test | Sudan black. Periodic acid Schiff’s. Iron staining. Leukocyte alkaline phosphate. Feulgen reaction. Muramidase reaction. |
||
Kleuhaeur Betke test | Uses. Techniques. | ||
Myeloproliferative disorders | Leukaemia. Non-leukaemia. |
||
Mylodysplastic Syndromes | Causes. Detection techniques. |
||
Project | Project proposal. Generating data. |
Topic | Sub-topic | ||
Systemic disorders | Lupus erythromatosus. Microangiopathy. Detection techniques. |
||
Multiple Myelona | Plasma cell leukaemia. Abnormal immunoglobulins. Detection techniques. |
||
Malignant Lymphomas |
Hodgkin's disease. Non Hodgkin's diseases. Burkettes lymphoma. |
||
Polycythaemia | Erythraemia Absolute. polycythaemia Relative. polycythaemia. | ||
Electronic Counters |
Mucipus. Operation. Prevention maintanance. |
||
Radioisotopes | Types. Uses. Safety precautions. |
||
Laboratory Animals | Types. Handling. disposal. |
||
Blood Parasites | Types. Significance. Detection techniques. |
||
Project | Data analysis. Project report. Write up and presentation. |
Topic | Sub-topic | ||
Introduction | Terminology used, relation of histopathology to other subjects, reception, handling of samples, microscopic appearance of body organs, source of samples. | ||
Cell and epithelial | Cell structure, cell division, four primary tissues, types and functions of epithelial cells, connective tissues, muscular tissue, nervous tissue, overview of body systems. | ||
Fixation and fixatives | Definitions, purposes, effects, terminologies used, charac- teristics, methods of fixation, storage of fixed tissues. | ||
Decalcification | Purpose, methods, tissues requiring decalcification, end points of decalcification, treatment of tissues after decalcification. |
Topic | Sub-topic | ||
Safety Measures | Fire hazards, physical and chemical injuries, explosives, implosives, infectious material. | ||
Histochemistry | Nature of enzymes, types, frozen section, freeze drying, freeze substitution, equipments used, demonstration techniques. | ||
Museum techniques | Collection, preservation, labelling, display, staining of gross specimens, photomicrography. | ||
Microscopy | Review of light microscope, principles of electron microscope differences, limitations of light and electron microscopes, preparation of electron microscopy samples, examination and interpretation. | ||
Mausoleum techniques | Government policy on bodies, reverence for the dead, medical legal cases, post-mortems, embalming, disposal of bodies, mausoleum design. | ||
Management | Human resources management, material resource management, monetary management, design of a histological laboratory. | ||
Quality control | Reagents, procedures, techniques. |
Topic | Sub-topic | ||
ABO blood group system | ABO antigens. ABO antibodies. Structure of A and B antigen and their synthesis. |
||
ABH blood group system | Precursor substance. H gene. Secretor. Non-sector. Bombay phenotype. Inheritance. Nomenclature. Rh antigen. Rh pull phenotype. |
||
Other blood group systems | Mnss, P, Xg, Kell Ii. Lewis, Dufy, Lutheran, Kidd and private blood group system. |
||
Auto-immune antibodies and non-specific antibodies | Warm antibodies. Cold antibodies. Conditions associated with autoimmune antibodies. |
||
Immuno-globulins | Types. Characteristics. Differentiation and identification techniques. Importance. |
Topic | Sub-topic | ||
Haemolytic disease of the new-born | ABO antibodies. Rh antibodies. Causes/aetiology. Pathogenesis. Laboratory investigation. Exchange transfusion. Intra-uterine blood transfusion. Admission of RhoGam. Amniocentesis. |
||
Blood transfusion reaction | Intravascular transfusion reaction. Extra vascular transfusion reaction. Febrile non-haemolytic reaction. Anaphylactic. Decreased red cell lifespan. Diagnostic techniques. G6PD deficiency. Management. Spherocytosis. |
||
Red cell hereditary conditions | Ellitocytosis. Stomatocytosis. Laboratory investigation. |
||
Blood donor centre | Planning. Setting. Recruitment of donors. Phlebotomy. Screening of donated blood. Maintaining cold chain maintenance. Disposal of contaminated blood. Safety and quality. Assurance in blood bank. First-aid. |
||
Blood products | Cryoprecipitate. Fresh frozen plasma. Platelet rich plasma. Platelet concentrate. Red cell concentrate. Neocytes. White cell concentrate. |
||
Preparation of reagents and antisera | Anticoagulants. Grouping antisera. Lectins. Enzymes. Bovine albumin. Anti human globulins. |
Topic | Sub-topic | ||||
Application of radioisotopes | Estimation of blood volume. Determination of transfused red survival rate. Safety precaution. |
||||
Blood group in forensic medicine | Medical-legal. | ||||
Management of blood transfusion centre | Human resources. Laboratory resources. Documentation. |
||||
National blood transfusion policy | Interpretation. Implementation. |
||||
Instrumentation | Automatic blood group analyser. Operation and preventive maintenance. |
Topic | Sub-topic | T | P | ||
Parasitology laboratory | Laboratory set up. Furnishing equipment and apparatus. Chemicals and reagents and stains. Laboratory safety and waste disposal. Other expendable items. |
||||
Host parasite interrelationship |
Evolution of human parasitic infection. Host selection by parasites. Adaption and establishment of parasites. Evasion mechanisms. Pathological effects of parasite on their hosts. Immunology of parasitic diseases. |
||||
Parasitological specimens | Types and special collection methods. Preservation and transportation. Processing and disposal. |
||||
Transmission and laboratory diagnosis |
Protozoan infections. Amoebiasis. Giardiasis. Trichomoniasis. Balantidiasis. Cryptosporidiosis. Isosporidiosis. Malaria. Leishmaniasis. Trypanosomiasis. |
Topic | Sub-topic | T | P | ||
Transmission and laboratory diagnosis | Protozoal infections. Toxoplasmosis. Babesiosis. Pneumocystosis. Sacocystosis. Acanthamoebiasis. Acanthaoeiasis. Amoebi meningoepicaphalitis. Other minor protozoal diseases. Cestodeal infections. Taeniasis. Cysticercosis. Hydatidosis. Sparaganosis. Diphyllobothriasis. Sparaganosis. Coenurosis. Hymenolepiasis. Other minor cestodeal infections. Trematodeal infections. Schistosomiasis. Fascioliasis. |
||||
Fasciolopsiasis. Paragonimiasis. Opisthorchiasis. Clonorchiasis. Heteropyiasis. Other minor trematodeal infections. Nematodeal infections. Ascariasis. Trichuriasis. Ancylostomiasis. |
Topic | Sub-topic | ||
Transmission and lab diagnosis | Nematodeal infections. Trichinellosis and trichinosis. Enterobiasis. Strongyloidiasis. Loiasis. Mansonelliasis. Bancoroftian filariasis. Brugian filariasis. Onchocerciasis. Dipetalonemiasis. Dracunculiasis. Capillariasis. Minor parasitic infections. Gnathostomiasis. Macracanthrorhynchiasis. Pentastomidiasis. Arthropod caused conditions. Myiasis. Scabies. Tungiasis. Pediculosis. Entomosis, entomophobia including delusory parasites. Paralysis (tick induced). Envenomisation, dermatitis. Sensitization and hypersensitivity. |
||
Vector indentification and incrimination | Special methods. Forensic entomology. |
||
Parasitological entomological surveys | Geographical reconnaissance. Baseline data collection on parasites and vectors. Vector bionomics. Forecasting of epidemics. |
||
Prevention and control | Epidemiology of parasitic disease. Anti-parasitic and anti-vector measures. Integrated approaches environmental considerations. Sensitivity and susceptibility testing. |
||
Monitoring and evaluation of control measures | Verifiable indicators. Means of verification. Inputs. Outputs. Impacts assessment. |
||
Revision, project compilation, examinations. |
Term | Topic | Sub-topic | T/P | Hours |
ONE. | Introduction | Definition of viruses. History of virology. Major developments and contributions in virology. |
||
Classification of viruses |
Criteria used for classification. Virus architecture— • structure, • components. Virus replication. Methods of studying viruses. |
|||
Bio-safety | Bio-safety procedures applied in Virology. Code of practice. Categorization of pathogens into risk groups. |
|||
Virus laboratory Categories and setting |
Tissue culture lab. Serology. Containment level – 3. Maximum containment level – 4. Animal house. Washing up/sterilization room. |
Sterilization disinfection and disposal | Sterilisation techniques. Physical. Chemical— • sterility testing, • disinfection and disinfectants, • disposal procedures. • Animals. • Materia. |
Term | Topic | Sub-topic | T/P | Hours |
TWO | Equipments | Principles and uses of the following equipment: Microscopes-Electron Microscope, inverted, If Refrigerators/deep freezers Containers for liquid nitrogen, Carbon dioxide etc. Incubators and autoclaves De-ionizers and distillers Bio-safety cabinets and flow Cytometer Lyophiliser pH Meters, Thermocycler |
||
Virological specimens | Containers. Specimen. Types: processing, preservation, storage, transportation. |
Term | Topic | Sub-topic | T/P | Hours |
Epidemiology of viral diseases |
Course of infection in an individual: Acute *latent *chronic. Routes of spread in the Community: *horizontal *vertical. Surveillance of viral diseases. |
|||
Pathogenesis | Virulence. Localised infections. |
|||
Systematic Virology | Generalised infections. Systemic infections. DNA viruses. RNA viruses. |
Term | Topic | Sub-topic | T/P | Hours |
THREE. | Systemic virology | Unclassified viruses. | ||
Tissue culture systems *1 degree *2 degree, continuous cell lines, cultivation, inoculation and harvesting. Animal techniques. Immunological/serological techniques *ELISA *IFA. Quantitation of virus litres. LDSO, TCID50, ELD59, PFU-RIA-CFT-RPHA-HAI-HAD-Immunoblot assays |
||||
Microbial genetics and molecular biology | DNA Cellular organisation and function. Gene mutation. Mechanisms of genetic exchange. Aspects of molecular biology. |
Term | Topic | Sub-topic | T/P | Hours |
Techniques used in molecular virology e.g. PCR and flowcytometry. | ||||
Control of viral diseases | Antiviral drugs *Target sites for antiviral drugs. *Mechanisms of action of antiviral. |
|||
(Antiviral therapy) (vaccines, others) | Drugs. Vaccines— *live attennated, *killed vaccines, *recombination vaccine, Large and small scale vaccine production. Vaccine storage and distribution. Other control methods of viral diseases— vector control, quarantine methods, surveillances. |
|||
Total quality management (Tqm) in virology | Definition of TQM. Quality control. Design and benefits of quality assurance. |
Topic | Sub-topic | T/P | Hrs | |
Term One 220 hours |
Introduction | Historical background. Early immunology. Recent advances of immunology. Development of immunological. Techniques. Chronology of important. achievements in immunology. |
||
Innate and acquired immunity | The innate immune system. The role of complement and phagocytic cells Soluble factors. The acquired immune system. The role of antibodies. *B Lymphocytes. *T Lymphocytes. *Phagocytic cells. |
|||
Biology of the immune system | The primary lymphoid organs. The secondary lymphoid organs. The lymphoid cells. *T & B Lymphocytes. *N K Cells. The mononuclear. Phagocytic system. Polymorphonuclear granulocytes platelets. |
70 | ||
4. | Biosafety | Bio-safety techniques in immunology laboratory. | 24 | |
5. | The major histo- compatibility complex |
The arrangement of MHC genes. Cellular distribution of MHC antigens. Structure of MHC antigens. Functions of the MHC antigens. The immuno-globulin supergene family. |
40 | |
Term Two (2) 320 Hours (T/P) 120 Hours Project |
Immunochemistry | The immuno globulin structure and functions. Classes and subclasses. The generation of diversity. Theories of antibody formation. Light chain and heavy chain gene. Selection recombination. Recombination sequences. Production of immuno globulin. |
7. | Complement system | The complement proteins. The classical pathway. The alternate pathway. The complement receptors. Complement associated diseases and deficiencies. |
99 | |
8. | Immunological techniques and instrumentation |
Agglutination. Precipitation. Production of anti-sheep RBC’s serum. Complement assays. Separation and purification of immunolobulins. Preparation of antisera purity check. Fluorescence techniques. |
||
ELISA. Hybridoma technology: Production of monoclonal antibodies. Electrophoresis & immuno-electrophoresis. PCR polymerase chain. Reaction.Blot immuno assays. Flow cytometry. |
Term Three (3) |
Hypersensitivity reactions | Type I Immediate hypersensitivity. Concept at allergic reactions. |
||
440 Hours 8 |
Type II ADCC. Antibody dependant. Cell cytoxity. Type III Complex mediated. Hypersensitivity mechanisms at damage. Type IV Delayed hypersensitivity. Conditions manifesting Type III. |
9. | Immuno deficiency states |
Definition 1 per cent Immunodeficiency 2 per cent Immunodeficiency Mechanisms leading to immuno- deficiency. Methods of investigating. |
||
10. | Auto immunity | Self tolerance. Breakdown at self tolerance. Immuno suppresion. Auto immune disorders. |
11. | Transplantation and rejection | Genetics of transplantation. Mechanisms of graft rejections. Clinical tissue— *Allogeneic. *Syngeneic. *Xenogeneic. Transplanatation. Immuno-suppression. Histo-compatibility testing. |
||
12. | Tumor immunology | Immune recognition of tumors. Immune responses to tumors (surveillance). Potential for therapy. Animals. Induction of tumors in laboratory. |
||
13. | Vaccines immunomodulators | Types of immunization. Types of vaccines and immunoi- modulators. Usage of vaccines. Vaccine storage and distribution. |
||
14. | Immunity to pathogens | Immunity to viruses. Immunity to fungi. Immunity to bacteria. Immunity to protozoa. Immunity to parasitic helminthes. |
||
15. | Total Quality Management |
Concepts of TQM. Benefits and design. |
Appendix: 1
TRAINING STANDARDS
1.Staff/Student RatioLecturersTheory 1:10Practical 1:52.Academic Staff QualificationsMinimum HD MLS with five (5) years experience in specialist subject.A certificate in, medical education, and good track record.orMSc. in Medical Laboratory Sciences (relevant subjects) and registered by KMLTTB.Support staff should comprise of—–One (1) HD Holder–One (1) Diploma Holder–One (1) Certificate Holder3.Attendance – 90%4.Duration of Programme – One (1) Year5.Distribution of Learning TimeTheory – 60%Practical – 40%6.Subject Taken – One at a given time7.Average Pass Mark – 50%8.External ExaminersHD holder with five (5) years working experience, medical education shall be an added advantage. Proven track record.M.Sc. and above in medical laboratory sciences with good reputation and integrity.9.Examination Declaration-Common examination shall be given to all students in the Republic of Kenya.-Examination results shall be declared soonest possible.-Supplementary examination shall be given three (3) months after the declaration of the final results.-Upon unsuccessful attempt of the supplementary, the candidate shall be referred for one (1) year.Appendix: 2
ESSENTIAL EQUIPMENT
MICROBIOLOGY
1. Autoclave (portable) | 1 between 10 students | ||
2. Medium water bath | 1 between 5 students | ||
3. pH meters | 1 between 5 students | ||
4. Anaerobic jars | 1 between 5 students | ||
5. Incubators/hot air oven (adjustable) | 1 between 10 students | ||
6. Distillers | 2 for the whole institute | ||
7. De-ionizers | two small | ||
8. Microscopes (Binocular) | 1 between 10 students | ||
9. Weighing balance | 1 top pan load balance | ||
10. Woods lamp | one | ||
11. Centrifuge | 1 between 4 students | ||
12. Bunsen Burner/Spirit | 1 between 2 students | ||
13. Tripod Stands/asbestos mat | 1 between 10 students | ||
14. Fridge/deepfreezer | 1 between 10 students | ||
15. Safety Cabinet | 1 per laboratory | ||
16. Teaching microscopes | 1 between 10 students | ||
17. Mechanical shaker | 1 between 10 students | ||
18. Inoculating loops | 1 per student | ||
19. Assorted microbiology glassware | adequate | ||
21. Food masseraurs | 1 between 5 students | ||
22. Colony counters | 1 between 5 students | ||
23. Nephelometer | |||
24. Compute | 1 per class | ||
25. Electrophoresis equipment | 1 per class |
CLINICAL CHEMISTRY
1. Colorimeters | 1 between 4 students | ||
2. Analytical balance – top pan loading | |||
3. Sensitivity up to 1 mg | 1 between 5 students | ||
4. Flame photometers | 1 between 10 students | ||
5. Centrifuge | 1 between 4 students | ||
6. Refrigerators/freezers | 1 between 10 students | ||
7. Water bath medium | 1 between 4 students | ||
8. pH meter | 1 between 5 students | ||
9. Mechanical mixers | 2} | ||
10. Electrophoresis equipment | 2} per institution/class | ||
11. Distiller/de-ionizer | 2} | ||
12. Hot air oven/incubator adjustable | 10 | ||
13. Flame photometer | 1 between 5 students | ||
14. Selective electrodes | two of them | ||
15. Electrophoresis equipment | 1 between 10 | ||
16. One auto-analyser | 1 per Institution/class | ||
17. Fluorimeter | 1–ditto | ||
18. Immunochemistry analyzer | 1–ditto | ||
19. Thermocycler for PCR | 1–ditto | ||
20. Blood gas analyzer | 1–ditto | ||
21. High pressure liquid chromatogram | (HPLC) | ||
22. Scintillation counter | 1–ditto | ||
23. Nephelometer | 1–ditto | ||
24. Computer | 1–ditto |
HAEMATOLOGY
1. Haemoglobinometers | |||
2. Centrifuge | –ditto– | ||
3. Microhaematocrit centrifuge | 1 between 5 students | ||
4. Microscopes – Blood mixers rollers | 1 between 10 students | ||
5. Water bath | –ditto– | ||
6. Incubator | –ditto– | ||
7. Colorimeter | –ditto– | ||
8. Electrophoresis equipment | 1 between 10 students | ||
9. Sphygmomanometer | 1 between 5 students | ||
10. E.S.R. stands | 1 between 4 students | ||
11. Deepfreezer/fridge | 1 per 10 students | ||
12. Deep freezer | 1 between 5 students | ||
13. Coulter Counter | 1 for each class | ||
14. Neubaeur Chambers | 1 for each student | ||
15. Distiller | 2 per institution/class | ||
16. Analytical balance | 1 between 10 students | ||
17. Stethoscopes | 1 between 5 students | ||
18. Spectroscope – Direct vision/revision | |||
19. Refrigerated centrifuge | 1 per institution | ||
20. Computer | " |
HISTOPATHOLOGY
1. Microtome (Rocking/Rotary) | 1 per 4 students | ||
2. Manual tissue processing set | 1 between 4 students | ||
3. Hot plate | 1 between 6 students | ||
4. Hone and strope | 1 between 4 students | ||
5. Automatic knife sharpener | 1 per class/institution | ||
6. Water bath, medium size | 1 between 4 students | ||
7. Microscope (teaching) | 1 for the institution | ||
8. Cold plate | 1 between 6 students | ||
9. Weighing balances | 1 between 1 students | ||
10. De-ionizers | 1 per class/institution | ||
11. Fume chambers | 1 per laboratory/institution | ||
12. Automatic tissue processor | 1 per class/institution | ||
13. Automatic staining machine | 1 per class/institution | ||
14. Freezing microtone – hard set | 1 per class/institution | ||
15. Centrifuge | |||
17. Computer | |||
18. Postmortem kit |
BLOOD TRANSFUSION SCIENCE
1. Blood bank refrigerator | 1 per class | ||
2. Grouping tiles | 1 per student | ||
3. Water bath | (medium size) adjustable | ||
4. Plasma extractors | 15 students | ||
5. Centrifuges | 1 between 4 students | ||
6. Weighing balance | 1 between 5 students | ||
7. Syphomomanometers | 1 between 5 students | ||
8. Hot air oven (adjustable) | 1 in the whole institution | ||
9. De-ionizers and stillers | 1 for the whole class/institution | ||
10. Mechanical shaker | 1 per 2 students | ||
11. Blood Transfusion bleeding unit | 1 per 2 students | ||
12. Assorted blood transfusion glassware and adequate apparatus | 1 per 2 students | ||
13. Microscopes | |||
14. Deep freezer 70 degrees C | |||
15. Automated centrifuge for blood products | |||
16. Cool boxes | |||
17. Microscopes | |||
18. Water bath |
MEDICAL PARASITOLOGY
1. Centrifuges | 1 for 4 students | ||
2. Refrigerators | –ditto– | ||
3. Pestle and mortar | 1 per student | ||
4. Teaching microscope | 1 per student | ||
5. QBC unit | 1 per student | ||
6. Assorted apparatus e.g. racks, test tubes, stirring rods, applicator sticks, forceps Funnels, Kato kits, hand lenses | 1 per lecturer | ||
7. Stereo microscope, one per class | |||
8. Fluorescent microscope, one per student | |||
9. Geiger Muller counter/scintillator | |||
10. Microscope, binocular, one per student | |||
11. Dissecting microscope, one per student | |||
12. Stereo microscopes, one per lecturer | |||
13. Refrigerated centrifuge | |||
14. Mosquito scoops | |||
15. Sucking tubes | |||
16. Slide boxes | |||
17. Dry specimen display tubes | |||
18. Traps for big mammals and small mammals | |||
19. Insect traps/tubes | |||
20. Ladles | |||
21. Mcmaster chambers | |||
22. Enamel tray | |||
23. Gumboots | |||
24. Knap sack/spray pumps | |||
25. Computer |
Appendix: 3
ESSENTIAL BOOKS
MICROBIOLOGY
Title | Author | ||
1. | A colour atlas of practical pathology and microbiology | Ramnia Sood | |
2. | A manual for laboratory and diagnostic tests | F. Fiscbaeh | |
3. | Clinical diagnosis and management | Method John B/Henry MD | |
4. | Clinical microbiology | J.J Inglis | |
5. | Fundamentals in microbiology | K.P. Talaro | |
6. | Medical immunology | Daniel/Tristram | |
7. | Microbiology (colour guide) | Inglis | |
8. | Basics of quality assurance for intermediate and peripheral laboratories | W.H.O | |
9. | Principles of bacteriology and immunology Vol. I, II and III | Topley and W ilson | |
10. | Handbook of bacteriology techniques | Baker | |
11. | Microbiology including Immunology and molecular genetics | B.D. David et al |
CLINICAL CHEMISTRY
Title | Author | ||
1. | Physiological Chemistry | Harper | |
2. | Clinical Chemistry Diagnosis and Treatment | P. Mayne et al | |
3. | Fundamentals of Clinical Chemistry | Norbert Teitz | |
4. | Practical Clinical Biochemistry (Vol. I and II) | Harold Varley | |
5. | Biochemistry: A Case Oriented Approach | Montgomery | |
6. | Biochemistry | Lehninger A.L. | |
7. | Quantitive inorganic Chemistry | H.Vogel | |
8. | Clinical Diagnosis | W.B. Saunders et al | |
9. | Clinical Chemistry | Henry | |
10. | The living Body | Best and Taylor |
HAEMATOLOGY
Title | Author | |||
1. | Clinical Haematology | Wintrobe | ||
2. | Practical Haematology | Dacie et al | ||
3. | Clinical Haematology in Medical Practice | De Crunchy | ||
4. | Leukaemia | F. Gruz | ||
5. | Leukaemia Diagnosis | Barbara Bain | ||
6. | Diseases of the Bone Marrow | Witby and Briton | ||
7. | Haematological Techniques for Medical Laboratory Technicians and Medical Students | Darmady and Davenport | ||
8. | Bleeding Disorders | Jardisty and Ingram | ||
9. | Haematology | William J. W illiam | ||
10. | Chronic Granulocytic Leukaemia | Michael T. Show |
HISTOPATHOLOGY
Title | Author | ||
1. | Understanding Pathophysiology | Sue E. Heuther, Kathryn L. McCain | |
2. | Carleton’s Histological Techniques | Dory and W ellington | |
3. | Practical Section Cutting and Staining | Clayton | |
4. | Cellular Pathology Technique | C.F.A. Culling et al | |
5. | Wheater’s Functional Histology a Textbook and Colour Atlas | Young/Heath | |
6. | Simpson’s Forensic Medicine | B Knight | |
7. | Mausoleum Techniques | ||
8. | Electron Microscopy | ||
9. | Cytology |
BLOOD TRANSFUSION
Title | Author | |||
1. | Haematology | William J. W illiam | ||
2. | Techniques in blood Grouping | Ivory Dunford and C.C. Bowky | ||
3. | Modern Blood Banking on Transfusion Services | D. Harmening and Pittiglio | ||
4. | Blood Grouping in Man | R.R. Rale and R. Sanger | ||
5. | Blood Transfusion in Clinical Medicine | Mollison Patrick | ||
6. | Blood Group Serology | I. Dnford and C.C. Bowky |
PARASITOLOGY
Title | Author | |||
1. | Textbook of Parasitology | Daniel L. Belding M.D | ||
2. | Clinical Parasitology | Craig and Faustin | ||
3. | Introduction to Parasitology | A.C. Chandler | ||
4. | Tropical Diseases | Manson Barr | ||
5. | Essential Marariology | Bruce Chwatts | ||
6. | Worms and Diseases | Muller R | ||
7. | Lecture Notes of Medical Entomology | M.W . Service | ||
8. | Insects of Medical Importance | |||
9. | Medical Parasitology | Markell, Vogue and John | ||
10. | Entomology for Students of Medicine | Gordore M.M.J. Laboipierne |
VIROLOGY
Title | Author | |||
1. | Practical Virology for Medical Students and Practitioners in tropical countries | D. Metasalaar et al | ||
2. | Fundamentals of Medical Virology | Kucera and Louis S. | ||
3. | Virological Procedures | Hopkins et al | ||
4. | Virology – Practical Approach | B.S. Mahy et al | ||
5. | Medical Virology | D. W hite & F. Ferner | ||
6. | Medical Virology – a Practical approach | Editor – U. Desselberger | ||
7. | Principles of molecular Virology | J Cann |
IMMUNOLOGY
Title | Author | |||
1. | The Principles of Immunology | Ivan Roitt | ||
2. | Fundamentals of Immunology | Tesdale | ||
3. | Practical Immunology | Hudsons and Hay | ||
4. | Practical Immunology | Talwar | ||
5. | Basic & Clinical Immunology | Peakman & Vergains | ||
6. | Understanding Immunology | Peak Woods & Prentice-Hall |